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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (30): 106-110.doi: 10.11924/j.issn.1000-6850.2011-3420

所属专题： 生物技术； 水稻

• 农学 农业基础科学 • 上一篇    下一篇

稻米留胚率与蛋白质含量的相关性研究

王嫘 王芳 王子怀 丁毅 张芹 华泽田   

  • 收稿日期:2011-11-17 修回日期:2012-01-11 出版日期:2012-10-25 发布日期:2012-10-25
  • 基金资助:

    高异交粳型不育系及强优势恢复系选育;粳型高异交两系不育系创制与亚种间杂种优势利用

Correlation Study on the Plumule Ratio and Protein Content of Rice

  • Received:2011-11-17 Revised:2012-01-11 Online:2012-10-25 Published:2012-10-25

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摘要：

为了研究稻米留胚率与蛋白质含量的相关性，以10个品种统一种植统一收获的水稻糙米为实验原料，设置不同的碾精时间，碾精后统计留胚率。用凯氏定氮法和考马斯亮蓝法测定糙米和不同碾精时间精米的蛋白质含量，研究留胚率与蛋白质含量的相关性，最后通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳比较各蛋白组分之间的差异。结果表明：不同粳米品种在同一碾精时间下留胚率不同，留胚率随碾精时间延长而下降；稻米碾精后的蛋白质含量与其留胚率相关，一般留胚率变化较小的品种在碾精后蛋白质损失少，但也有例外；另外，随着碾精时间延长，谷蛋白和醇溶蛋白占总蛋白比例上升，清蛋白和球蛋白比例下降。

关键词: 超高产, 超高产

Abstract:

To investigate the correlation between the plumule ratio and protein content of rice, the brown rice of ten varieties which were uniform planting and harvesting was used as the experimental materials. Plumule ratio of the milled rice with different milled time was counted. Then the protein content of the brown rice and milled rice with different milled time was measured by Kjeldahl determination and Bradford determination to study the correlation of plumule ratio and protein content. Finally, the differences were compared between various protein components of the varieties with SDS-PAGE analysis. The results showed that the plumule ratio was different between different varieties and decreased with prolonging the milled time. The protein content has correlation with the plumule ratio of milled rice. Generally the protein content of milled rice loses less if the plumule ratio changes slightly, although the exception existed. Besides, the percentage of glutelin and gliadin increased and albumin and globulin decreased with the extension of milled time.