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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (8): 75-79.doi: 10.11924/j.issn.1000-6850.2012-3230

所属专题： 水产渔业

• 水产 渔业 • 上一篇    下一篇

磺胺类药物胶体金快速检测试剂条的研制及在水产品中的应用

张敏 李翘 盛慧萍   

  • 收稿日期:2012-09-24 修回日期:2012-10-29 出版日期:2013-03-15 发布日期:2013-03-15

Development and Application of Colloidal Gold Rapid Test Strip for Sulfonamides in Aquatic Products

  • Received:2012-09-24 Revised:2012-10-29 Online:2013-03-15 Published:2013-03-15

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摘要： 为建立一种快速检测水产品中磺胺类药物残留含量的检测方法，应用胶体金免疫层析技术，研制了一种水产品中磺胺类药物免疫胶体金快速检测试剂条。将羊抗鼠IgG、免疫抗原(SDL-HSA)和金标抗体三者经过反复调试优化，以适宜浓度和包被量分别包被到硝酸纤维素(NC)膜和胶体金结合垫上，包被后的硝酸纤维素(NC)膜、样品垫、胶体金结合垫、吸水垫及其他辅料组装成试剂条。结果表明：该试剂条对水产品中8种磺胺类药物：磺胺嘧啶(SD)、磺胺噻唑(ST)、磺胺甲基异恶唑(SMZ)、磺胺间甲氧嘧啶(SMM)、磺胺二甲基嘧啶(SM2)、磺胺喹恶啉(SQX)、磺胺甲氧哒嗪(SMP)、磺胺对甲氧嘧啶(SMD)的检出限分别为5、5、10、40、45、50、100、100μg/kg，与链霉素、四环素、氯霉素、盐酸克伦特罗、环丙沙星等其他兽药无交叉反应。试验证明检测试剂具有较高的灵敏度及特异性、操作便捷、稳定可靠，可作为磺胺类药物残留检测的一种快速简便方法。

关键词: 再生体系, 再生体系

Abstract: To establish a rapid detection method of sulfonamides residues in aquatic products. Application the GICA technology developed a rapid detection test strip of sulfonamides in aquatic products. Goat anti-mouse IgG, Immunogen antigen (SDL-HSA) and gold labeled monoclonal antibody three after repeated testing optimized to suitable concentration and spray volume coated to nitrocellulose (NC) membrane and micropore. The test indicated that: the lowest detection limits of SD, ST, SMZ, SMM, SM2, SQX, SMP, SMD of eight kinds of Sulfonamides are 5, 5, 10, 40, 45, 50, 100, 100μg/kg. No cross-reactivity with other veterinary drugs such as streptomycin, tetracycline, chloramphenicol, clenbuterol, ciprofloxacin. The result showed that the test device has high sensitivity and specificity and was easy to operate. It could be the most rapid and convenient means for detection of sulfonamides in the aquatic products.