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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (4): 48-53.doi: 10.11924/j.issn.1000-6850.casb16060123

• 林学 园艺 园林 • 上一篇    下一篇

欧美杨(Populus nigra×P. deltoides)再生体系的建立

张瑞芝,王 峰,李丹蕾,陈俏丽,牛春阳,零雅茗   

  1. 东北林业大学林学院,东北林业大学林学院,东北林业大学林学院,东北林业大学林学院,东北林业大学林学院,东北林业大学林学院
  • 收稿日期:2016-06-24 修回日期:2017-01-18 接受日期:2016-09-20 出版日期:2017-02-10 发布日期:2017-02-10
  • 通讯作者: 李丹蕾
  • 基金资助:
    国家自然科学(31500524)；中央高校基本科研业务费专项资金项目(2572015EY02,2572015CA09)；黑龙江省博士后基金(LBH-Z11248)；高等学校博士学科点专项科研基金(博士点新教师基金20100062120002)资助。

Establishment of Populus nigra× P. deltoides Regeneration System

张瑞芝,,,, and   

  • Received:2016-06-24 Revised:2017-01-18 Accepted:2016-09-20 Online:2017-02-10 Published:2017-02-10

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514

摘要： 黑杨派（Aigeiros）的杂交后代欧美杨（Populus nigra × P. deltoides）在杨树叶锈病研究领域具有重要价值。本研究以叶柄、叶片和茎段作为外植体进行组织培养，构建欧美杨再生体系。分别优化了初代培养、继代培养、抽茎培养和生根培养的培养条件。正交试验结果表明最优初代培养培养基配方：MS 0.5 mg/L 6-BA 0.05 mg/L NAA。最优继代培养培养基配方：MS 0.5 mg/L 6-BA 0.1 mg/L NAA。最优抽茎培养培养基配方：MS 0.1 mg/L 6-BA 0.05 mg/L NAA。最优生根培养培养基配方：1/2MS 0.25 mg/L NAA 20 g/L蔗糖。以上培养基培养分别得到叶柄分化率为86.7%，不定芽诱导率为96.7%，抽茎率为91.7%，生根率为85%。再生苗移栽成活率为91.5%。所获得的欧美杨再生苗扩繁简便，可用于杨树抗叶锈病相关基因转化等研究。欧美杨再生体系为揭示欧美杨的感病机理提供物质基础。

关键词: 苹果腐烂病菌, 苹果腐烂病菌, 杀菌剂, 生物药剂, 抑菌率

Abstract: The Populus nigra × P. deltoides, the hybrid offspring of Aigeiros, is useful in the pathology of poplar against the infection of rust. The petiole, leaves and stem segments were cultured as explants to induce seeding. Optimal conditions of the regeneration system were determined for the primary culture, subculture, stem elongation culture and rooting culture. While the best primary culture medium (MS 0.5 mg/L 6-BA 0.05 mg/L NAA) was used, 86.7% of petioles transformed to seedlings. Further more, an adventitious bud induction rate of 96.7% was got when the subculture medium (MS 0.5 mg/L 6-BA 0.1 mg/L NAA) was adopted. A stem elongation rate of 91.7% was got when the stem culture medium (MS 0.1 mg/L 6-BA 0.05 mg/L NAA) was used. While the root media (1/2MS 0.25 mg/L NAA 20 g) was used, 85% of the seedlings were rooted. The survival rate of the regenerated seedlings was 91.5%. The Populus nigra × P. deltoides regeneration system can be rapidly propagated and used in gene inheritance and transformation. It also can provide material basis for the study of poplar susceptibility/resistance to the rust fungi.

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