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中国农学通报 ›› 2013, Vol. 29 ›› Issue (30): 41-44.doi: 10.11924/j.issn.1000-6850.2012-3887

所属专题: 生物技术 小麦

• 农学 农业基础科学 • 上一篇    下一篇

小麦-黑麦二体代换系间杂交诱导染色体易位的研究

李宇欣 张利国 厉永鹏 张延明   

  • 收稿日期:2012-11-30 修回日期:2013-01-23 出版日期:2013-10-25 发布日期:2013-10-25

Induced Chromosome Translocation by Hybridized between Wheat-Rye Disomic Substitution Lines

  • Received:2012-11-30 Revised:2013-01-23 Online:2013-10-25 Published:2013-10-25

摘要: 摘 要:小麦-黑麦二体代换系间杂交,是诱导小麦-黑麦染色体易位的重要途径,有理论与应用价值。本研究利用DS1R/1D与DS5R/5A、DS6R/6A杂交,结果表明,F1减数分裂有19个二价体、4个单价体,有部分同源染色体配对和染色体易位现象;F2 对带有黑麦性状的24株普通小麦类型植株进行原位杂交检测,共检测出10株有染色体易位,易位频率为41.6%。易位株系为:06-16-7、06-16-3、06-16-5、06-16-7、06-16-8、06-17-7、0617-11、06-17-15、06-17-16、06-18-5。由于亲本选配、检测植株有一定的目的性,易位植株均表现有一定的应用价值。

关键词: 对策建议, 对策建议

Abstract: Abstract: Hybridization between Wheat-rye disomic substitution is an important way to induce wheat-rye translocation, which has important theory value and practical significance. In this study, DS1R/1D and DS5R/5A, DS6R/6A was hybridized. The results showed that F1 had 19 bivalents and 4 univalents in meiosis, homoeologous chromosome pairing and chromosome translocation phenomenon appeared;In F2,24 common wheat types with rye traits plants were detected by genome in situ hybridization and obtained 10 plants with chromosomal translocation, and the translocation frequency was 41.6%. Translocation lines was 06-16-7,06-16-3,06-16-5,06-16-7,06-16-8,06-17-7,0617-11,06-17-15,06-17-16 and 06-18-5,respectively. Because parent selection and testing plant had a definite purpose, translocation plants could have a practical applications.

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