尼罗罗非鱼MHC IIB基因多态性及其与链球菌病抗性的关系

doi:10.11924/j.issn.1000-6850.2013-1164

摘要/Abstract

摘要： 为了解MHC IIB基因多态性与尼罗罗非鱼链球菌病抗性的相关关系，本研究用2b-snp-sf和2b-snp-sr引物分别从尼罗罗非鱼40尾感病个体和40尾抗病个体的基因组DNA中扩增MHC IIB基因片段，扩增产物长度为775~797 bp。在775~797 bp核苷酸序列中，抗病群体的多态核苷酸位点有265个(33.2%)，感病群体中有255个(31.9%)。在该扩增片段编码的101个氨基酸位点中，抗病群体与感病群体分别有32个和33个多态位点。对80个个体的441个阳性克隆测序，发现有7种不同的MHC IIB等位基因主型，编码7个不同的氨基酸序列。大部分等位基因是两个群体共有的。等位基因Orni-DAB*0201和Orni-DAB*0401在抗病群体中出现的频率(15.5%、5.6%)显著高于在感病群体中出现的频率(7.4%、1.8%)，而等位基因Orni-DAB*0501和Orni-DAB*0601只出现在抗病群体。抗病群体中70%的个体出现2~4个等位基因，30%的个体出现1个等位基因；感病群体中42.5%的个体出现2~4个等位基因，57.5%的个体只出现1个等位基因。这提示个体中等位基因数量的多少与其对链球菌病的抵抗力有着明显的关系。个体中具有的MHC IIB等位基因数越多、抗病力越强。本研究结果为抗病尼罗罗非鱼选育奠定了基础。

关键词: 抗性, 抗性

Abstract: In order to understand the relationship between polymorphism of the major histocompatibility complex genes and the disease resistance of Nile tilapia, the MHC IIB gene fragment was amplified from 40 susceptible individuals and 40 resistant individuals, using primer pairs of 2b-snp-sf and 2b-snp-sr. The length of the PCR products was 775 to797 bp. Among the PCR products of 775 to 797 bp nucleotides acid, 265 (33.2%) nucleotide positions were variable in resistant individuals and 255 (31.9%) in susceptible individuals, respectively. Among the putative 101 amino acid sequence encoded by the amplification product, 32 amino acid positions were polymorphic in resistant individuals and 33 in susceptible individuals, respectively. 7 different MHC IIB alleles which encoded 7 different amino acid sequences were identified among 441 clones from 80 individuals. Most alleles were commonly found in both individuals. Alleles Orni-DAB*0201 and Orni-DAB*0401 were significantly more prevalent in resistant individuals than in susceptible individuals, with 15.5%, 5.6% in resistant stock and 7.4%, 1.8% in susceptible stock respectively. Allele Orni-DAB*0501 and Orni-DAB*0601 were only found in resistant individuals. 70% in resistant individuals presented 2-4 alleles, only 30% individuals presented 1 allele. Whereas 42.5% in susceptible individuals presented 2-3 alleles, 57.5% individuals presented 1 allele. These results indicated that individuals possessed more MHC IIB alleles have stronger resistance ability to streptococcus disease. This study has identified potential candidate genes for further investigation into the breeding of disease-resistant strain of Nile tilapia.

高风英庞纪彩卢迈新朱华平可小丽刘志刚. 尼罗罗非鱼MHC IIB基因多态性及其与链球菌病抗性的关系[J]. 中国农学通报, 2014, 30(2): 76-83.

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