禾本科主要作物抗旱相关基因及转基因研究进展

doi:10.11924/j.issn.1000-6850.2013-3108

摘要/Abstract

摘要： 干旱是限制作物生长和产量的重要因素之一，为提高禾本科主要作物水稻、小麦、玉米、甘蔗的抗旱能力，本研究从植物抗旱机理出发，归纳了植物抗旱相关的功能基因、转录因子以及信号因子。综述了水稻、小麦、玉米、甘蔗抗旱相关基因挖掘以及抗旱转基因的研究进展，针对今后作物抗旱性的研究，提出了开展逆境植物重要抗旱基因资源挖掘、构建多基因共表达系统以及开展科学、标准的转基因抗旱鉴定指标评价体系研究的建议。

关键词: 籽粒产量, 籽粒产量

Abstract: The ability of plants to tolerate drought conditions is crucial for normal plant growth and productivity. In order to improve the ability of graminaceous crops to tolerate drought, the functional genes, transcription factors, and the signal factor which was related with plant response to drought were summarized based on the plant drought resistance mechanism. Advances of drought-resistant genes and drought-resistant transgenic about graminaceous crops, such as rice, wheat, maize and sugarcane were reviewed. In addition, the suggestions about plant drought resistance research were made, including important drought resistant gene excavation in adversity plants, integrated expression system construction and scientific and standard evaluation system research on drought resistance of transgenic plant.

邢淑莲姚艳丽刘洋. 禾本科主要作物抗旱相关基因及转基因研究进展[J]. 中国农学通报, 2014, 30(18): 251-258.

参考文献

[1] 肖大伟,陈志钢.最近 30年水旱灾害对中国大陆地区粮食减产的影响分析[J].东北农业大学学报:社会科学版,2012,10(4):6-9.
[2] Xu D, Duan X, Wang B, et al. Expression of a Late Embryogenesis Abundant Protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice[J]. Plant Physiol,1996(110):249-257.
[3] Blum A. Drought resistance, water- use efficiency, and yield potential -are they compatible, dissonant, or mutually exclusive?[J]. Australian Journal of Agricultural Research,2005,56(11):1159-1168.
[4] Yang A, Dai X Y, Zhang W H. A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice[J].Journal of Experimental Botany, 2012, 63(7):2541-2556.
[5] Quan R, Hu S J, Zhang Z L, et al. Overexpression of an ERF transcription factor TSRF1 improves rice drought tolerance[J].Plant Biotechnology Journal,2010,8(4):476-488.
[6] He C M, Zhang W W, Gao Q, et al. Enhancement of drought resistance and biomass by increasing the amount of glycine betaine in wheat seedlings[J]. Euphytica, 2011,177(2):151-167.
[7] Gao S Q, Chen M, Xia L Q, et al. A cotton (Gossypium hirsutum) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat [J]. Plant Cell Rep 2009,28 (2): 301-311.
[8] Alia, Saradhi P, Mohanty P. Involvement of proline in protecting thylakoid membranes against free radical-induced photodamage[J]. Journal of Photochemistry and Photobiology B: Biology,1997,38(2): 253-257.
[9] 许祥明,叶和春,李国凤.脯氨酸代谢与植物抗渗透胁迫的研究进展[J].植物学通报,2000,17(6):536-642.
[10] Sakamoto A, Murata N. The role of glycine betainein the protection of plants fromstress: clues from transgenic plants[J]. Plant, Cell and Environment,2002(25):163-171.
[11] Munns R. Physiological processes limiting plant growth in saline soils: some dogmas and hypotheses[J]. Plant Cell and Environment, 1993(16):15-24.
[12] Tyerman S D, Niemietz C M, Bramley H. Plant aquaporins: multifunctional water and solute channels with expanding roles[J]. Plant, Cell and Environment,2002(25):173-194.
[13] 丰锦,陈信波.抗逆相关 AP2/EREBP转录因子研究进展[J].生物技术通报,2011(7):1-7.
[14] Nakano T, Suzuki K, Fujimura T, et al. Genome wide analysis of the ERF gene family in Arabidopsis and rice[J]. Plant Physiology, 2006,140(2):411-432.
[15] Wurschum T, Gross- Hardt R, Laux T. APETALA2 regulates the stem cell niche in the Arabidopsiss hoot meristem[J]. The Plant Cell, 2006,18(2):295-307.
[16] Guo A, He K, Liu D, et al. DATF: a database of Arabidopsis transcriptionfactors[J]. Bioinformatics,2005,21(10):2568-2569.
[17] 刘淑君,万红建,叶青静,等.植物 MYB转录因子的研究进展[J].分子植物育种,2011(9):1835-1842.
[18] 路子显,常团结,刘翔,等.植物碱性亮氨酸拉链(bZIP)蛋白的研究进展(一)[J].遗传,2001(23):564-570.
[19] 李伟,韩蕾,钱永强,等.植物 NAC转录因子的种类、特征及功能[J].应用环境生物学报,2011,17(4):596-606.
[20] Olsen A N, Ernst H A, Leggio L L, et al. NAC transcription factors: structurally distinct, functionally diverse[J]. Trends in Plant Sci, 2005,10(2):79-87.
[21] 韩冰,杨吉力,王艳芳,等.植物对干旱胁迫响应分子机制的研究进展[J].内蒙古大学学报:自然科学版,2006,37(2):227-232.
[22] Ingram J, Chandler J W, Gallager L, et a1. Analysis of cDNA clones encoding sucrose- phosphate synthase in relation to sugar interconversions associated with dehydration in the resurrection plant Craterostigma plantagineum Hochst[J]. Plant Physiology,1997 (115):113-121.
[23] Li H W, Zang B S, Deng X W, et a1. Over expression of the trehalose- 6- phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice[J]. Planta,2011,234(5):1007-1018.
[24] Dubouzet J G., Sakuma Y, Ito Y, et al. OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought- , high- salt- and cold- responsive gene expression[J]. Plant Journal,2003,33(4):751-763.
[25] Tian X H, Li X P, Zhou H L, et al. OsDREB4 genes in rice encode AP2- containing proteins that bind specifically to the dehydrationresponsive element[J]. Acta Botanica Sinica,2005,47(4):467-476.
[26] Dai X Y, Xu Y, Ma Q B, et al. Over expression of an RIR2R3MYBGene, OsMYB3R- 2, increases tolerance to freezing, drought, and salt stress in transgenic Aiabidopsis[J]. Plant physiology,2007(143):1739-1751.
[27] Chen H, Chen W, Zhou J L, et al. Basic leucine zipper transcription factor OsbZIP16 positively regulates drought resistance in rice[J]. Plant Science,2012(193-194):8-17.
[28] Huang X Y, Chao D Y, Gao J P, et al. A previously unknown zinc finger protein, DST, regulates drought and salt tolerance in rice via stomatal aperture control[J]. Genes & Development,2009,23(15): 1805-1817.
[29] Jeong J S, Kim Y S, Baek K H, et al. Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions[J]. Plant Physiology,2010,153(1):185-197.
[30] Xiong L Z, Yang Y N. Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acidinducible mitogen-activated protein kinase[J]. The Plant Cell,2003, 15(3):745-759.
[31] Saijo Y, Hata S, Kyozuka J, et al. Over-expression of a single Ca2+- dependent protein kinase confers both cold and salt/drought tolerance on rice plants[J]. The Plant Journal,2000,23(3):319-327.
[32] Ouyang S Q, Liu Y F, Liu P, et al. Receptor- like kinase OsSIKl improves drought and salt stress tolerance in rice (Oryza sativa) plants[J]. The Plant Journal,2010,62(2):316-329.
[33] Xu C Y, Jing R L, Mao X G, et al. A Wheat (Triticum aestivum) protein phosphatase 2A catalytic subunit gene provides enhanced drought tolerance in Tobacco[J]. Annals of Botany,2006,99(3):439- 450.
[34] 郭志爱,臧庆伟,景蕊莲,等.小麦小 G 蛋白 Rab2 基因 TaRab2 的克隆及其表达分析[J].作物学报,2007,33(2):201-207.
[35] 王转,臧庆伟,郭志爱,等.小麦幼苗期水分胁迫所诱导基因表达谱得初步分析[J].遗传学报,2004,31(8):842-849.
[36] 王爱萍,毛新国,景蕊莲,等.TaMyb2-Ⅱ基因在普通小麦(Triticum aestivum L.)及其近缘种中的氮核苷酸多态性分析[J].作物学报, 2006,32(12):1809-1816.
[37] Mahdi R, Xue G P, et al. A MYB gene from wheat (Triticum aestivum L.) is up- regulated during salt and drought stresses and differentially regulated between salt-tolerant and sensitive genotypes [J]. Plant Cell Reports,2010,29(8):835-844.
[38] Tong S M, Ni Z F, Peng H R, et al. Ectopic overexpression of wheat TaSrg6 gene confers water stress tolerance in Arabidopsis[J]. Plant Science,2007,172(6):1079-1086.
[39] 高世庆.大豆、小麦抗逆相关Gm/Ta AREB转录因子基因、启动子克隆及功能鉴定[D].北京:中国农业科学院,2007.
[40] Tian S J, Mao X G, Zhang H Y, et al. Cloning and characterization of TaSnRK2.3, a novel SnRK2 gene in common wheat[J]. Journal of Experimental Botany,2013,64(7):2063-2080.
[41] Xue G P, Heather M W, et al. Overexpression of TaNAC69 leads to enhanced transcript levels of stress up- regulated genes and dehydration tolerance in bread Wheat[J]. Molecular Plant,2011,4(4): 697-712.
[42] Zang Q W, Wang C X, Li X Y, et al. Isolation and characterization of a gene encoding a polyethylene glycol-induced cysteine protease in common wheat[J]. Journal of Biosciences,2010,35(3):379-388.
[43] Li A L, ZhuY F, Tan X M, et al. Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.)[J]. Plant Molecular Biology,2008,66(4):429- 433.
[44] 苏治军,郝转芳,谢传晓,等.玉米dbf1基因与耐旱相关性状的关联分析[J].植物遗传资源学报,2010,11(4):474-478,482.
[45] Sonia C, Cristina Peris- Peris, Laura M, et al. Expression of the maize ZmGF14- 6 gene in rice confers tolerance to drought stress while enhancing susceptibility to pathogen infection[J]. Journal of Experimental Botany,2011,63(2):983-999.
[46] Liu S X, Wang X L, Wang H W, et al. Genome-wide analysis of ZmDREB genes and their association with natural variation in drought tolerance at seedling stage of Zea mays L[J]. PLOS Genetics,2013,9(9):1-17.
[47] Ying S, Zhang D F, Fu J, et al. Cloning and characterization of a maize bZIP transcription factor, ZmbZIP72, confers drought and salt tolerance in transgenic Arabidopsis[J]. Planta,2012,235(2):253- 266.
[48] Huang C M, Yang L T, Li Y R, et al. Isolation and characterization of a gene encoding the Δ1- pyrroline- 5- carboxylate synthetase in sugarcane (Saccharum officinarum L.) [J]. Guangxi Agricultural Sciences,2009,40(2):113-116.
[49] 张积森,陈由强,郭春芳,等.甘蔗水分胁迫响应的Δ-鸟氨酸转氨酶基因克隆及分子特征分析[J].热带作物学报,2009,30(8):1062- 1068.
[50] 张积森,郭春芳,王冰梅,等.甘蔗水分胁迫响应相关醛脱氢酶基因的克隆及其表达特征分析[J].中国农业科学,2009,42(8):2676- 2685.
[51] Nogueira F T S, Schl?gl P S, Camargo S R, et al. SsNAC23, a member of the NAC domain protein family, is associated with cold, herbivory and water stress in sugarcane[J]. Plant Science,2005(169): 93-106.
[52] Trujillo L E, Menéndez C, Ochogavía M E, et al. Engineering drought and salt tolerance in plants using SodERF3, a novel sugarcane ethylene responsive factor[J]. Biotecnología Aplicada, 2009,26(2):168-171.
[53] 刘金仙,阙友雄,郭晋隆,等.甘蔗 S-腺苷蛋氨酸脱羧酶基因 ScSAMDC 的克隆和表达分析[J].中国农业科学,2010,43(7):1448- 1457.
[54] Babu R C, Zhang J X, Blum A, et al. HVA1, a LEA gene from barley confers dehydration tolerance in transgenie rice (Oryza asativa L.) via cellmembrane Protection[J]. Plant Science,2004 (166):855-862.
[55] Rohila J S, Jain R K, Wu R. Genetic improvement of Basmati rice for salt and drought tolerance by regulated expression of a bariey Hva1 cDNA[J]. Plant Science,2002(163):525-532.
[56] Jang I C, Oh S J, Seo J S, et al. Expression of a bifunetional fusion of the Eseheriehia coli genes for trehalose- 6- phosphate synthase and trehalose- 6- phosphate phosphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth[J]. Plant Physiology,2003(131):516-524.
[57] Benze X, Yuemin H, Ning T, et al. Over-epression of a LEA gene in rice improves drought resistance under the field conditions[J]. Theoretical and Applied Genetics,2007,115(1):35-46.
[58] Hu H, Dai M, Yao J, et al. Over expressing a NAM, ATAF, and CUC (NAC) transeription factor enhances drought resistance and salt tolerance in rice[J]. ProC. Natl. Aead. Sci. USA,2006,103(35): 12987-12992.
[59] Capell T, Escobar C, Liu H, et al. Overexpression of the oat arginine decarboxylase cDNA in transgenic rice (Oryza sativa L.) affects normal development patterns in vitro and results in putresine accumulation in transgenic plants[J]. Theor Appl. Genet.,1998(97): 246-254.
[60] Yang W, Kong Z, Omo- Ikerodah E, et al. Calcineurin B- like interaeting protein kinase OsCIPK23 functions in pollination and drought stressres in rice (Oryza sativa L.)[J]. J.Genet.Genom,2008 (35):531-543.
[61] Su J, Hirji R, Zhang L, et al. Evaluation of the stress- inducible production of choline oxidase in transgenic rice as a strategy for producing the stress- protectant glycine betaine[J]. Journal of Experimental Botany,2005,57(5):1129-1135.
[62] 刘伟华,赵秀振,梁虹,等.枯草杆菌果聚糖蔗糖酶基因转化小麦的研究[J].中国农业科学,2006(39):231-236.
[63] 郭北海,张艳敏.甜菜碱醛脱氢酶(BADH)基因转化小麦及其表达[J].植物学报:英文版,2000(42):279-283.
[64] Sivamani E, Bahieldin A, Wraith J M, et al. Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene [J]. Plant Science,2000(155):1-9.
[65] Vendruscolo E C, Schuster I, Pileggi M, et al. Stress- induced synthesis of proline confers tolerance to water deficit in transgenic wheat[J]. Journal of plant physiology,2007(164):1367-1376.
[66] Pellegrineschi A, Reynolds M, Pacheco M, et al. Stress- induced expression in wheat of the Arabidopsis thaiiana DREB1A gene delays water stress symptoms under greenhouse conditions[J]. Genome,2004(47):493-500.
[67] Dong C L, Zhang M Y, Zhang Y Q, et al. Transformation of trehalose synthase gene (TPS Gene) into corn inbred line and dentification of drought tolerance[J]. African Journal of Biotechnology,2011,10(68):15253-15258.
[68] Wei A Y, He C M, Li B, et al. The pyramid of transgenes TsVP and BetA effectively enhances the drought tolerance of maize plants[J]. Plant Biotechnology Journal,2010,9(2):216-229.
[69] 刘祥久,姜敏,张喜华,等.用开苞导入法将抗旱耐盐基因导入玉米自交系的初步研究[J].杂粮作物,2005(3):134-135.
[70] Zhang S J, Li N, Gao F, et al. Over- expression of TsCBF1 gene confers improved drought tolerance in transgenic maize[J]. Molecular Breeding,2010,26(3):455-465.
[71] Shou H X, Bordallo P, Wang K. Expression of the Nicotiana protein kinase (NPK1) enhanced drought tolerance in the transgenic maize1 [J]. Journal of Experimental Botany,2004(55):1013-1019.
[72] Zhang S Z, Yang B P, Feng C L. et al. Expression of the Grifola frondosa trehalose synthase gene and improvement of droughttolerance in sugarcane (Saccharum officinarum L.) [J]. Journal of Integrative Plant Biology,2006(48):453- 459.
[73] Molinari H, Marura C, Darosb E, et al. Evaluation of the stressinducible production of proline in transgenic sugarcane (Saccharum spp.): osmotic adjustment, chlorophyll fluorescence and oxidative stress[J]. Physiologia Plantarum,2007,130(2):218-229.
[74] Wu Y, Zhou H, Que Y X, et al. Cloning and identification of promoter Prd29A and its application in sugarcane drought resistance [J]. Sugar Tech,2008,10(1):36-41.