欢迎访问《中国农学通报》,

中国农学通报 ›› 2014, Vol. 30 ›› Issue (21): 136-140.doi: 10.11924/j.issn.1000-6850.2013-3411

• 农学 农业基础科学 • 上一篇    下一篇

一株胶质芽孢杆菌解磷活性及其适宜解磷条件研究

张伟伟 王宝琴   

  • 收稿日期:2013-12-30 修回日期:2014-03-23 出版日期:2014-07-25 发布日期:2014-07-25
  • 基金资助:
    滨州学院科研基金项目

Study on Phosphate-solubilizing Activity and Suitable Conditions of a Strain of Bacillus mucilaginosus

  • Received:2013-12-30 Revised:2014-03-23 Online:2014-07-25 Published:2014-07-25

摘要: 为研究一株胶质芽孢杆菌的解磷活性及适宜解磷的培养条件,在胶质芽孢杆菌的液体培养基中添加难溶性的磷酸钙培养7天后,采用钼锑抗分光光度法测定培养基中可溶性磷的含量,以稀释平板计数法测定菌体浓度,分析培养基中碳源、氯化钠浓度、pH值和菌体浓度对解磷活性的影响。结果表明:以葡萄糖作为碳源、0.03% NaCl、pH 6.0时该株胶质芽孢杆菌解磷活性最高,而菌体浓度对解磷活性无显著影响。

关键词: 纯化, 纯化

Abstract: In order to study a strain of Bacillus mucilaginosus’s phosphate-solubilizing activity and its suitable culture conditions for phosphate-solubilizing, Bacillus mucilaginosus was cultured for 7 days in the liquid medium added with lowly soluble calcium phosphate. The content of soluble phosphate in the medium was determined by the means of Molybdenum-Antimony Anti-Spectrophotometric method, and the concentration of cultured cell was measured using dilution plate counting. The effects of carbon source, NaCl concentration, pH value and cell concentration on phosphate-solubilizing activity were analyzed. The results showed that, the phosphate-solubilizing activity of Bacillus mucilaginosus reached the peak when the medium was supplied with the glucose (as carbon source) and 0.03% NaCl in a pH value of 6.0. However the cell concentration had no significant effects on phosphate-solubilizing activity .