关键词: 甜玉米, 甜玉米, 新品种, 农艺性状, 经济性状

Abstract: The aim is to study the purification technology of procyanidins from perilla seed hull and the antioxidant activity in vitro. The optimal purification parameters of procyanidins were obtained through static and dynamic experiments by using macroporous adsorption resin method. The antioxidant activity of procyanidins against DPPH and ABTS free radicals were evaluated by spectrophotometry. The results showed that XDA-8 was the best resin for purifying procyanidins in perilla seed hull, the adsorption rate was 63.41%, and the desorption rate was 78.98%. The optimal parameters were as follows: sample flow rate of 4 BV/h, sample concentration of 4 mg/mL, ethanol concentration of 70%, and elution flow rate of 4 BV/h. Under these conditions, the purity of procyanidins increased from 5.25% to 12.10%. The half-inhibition concentration (IC50) value of DPPH radical and ABTS radical scavenging activities for purified procyanidins was 2.138 μg/mL and 0.3699 μg/mL, respectively, and the scavenging abilities of purified procyanidins were higher than that of Vc. Therefore, XDA-8 resin purification method is simple and efficient, and can be used for the purification of procyanidins from perilla seed hull, and the purified procyanidins of perilla seed hull have stronger antioxidant activity in vitro.