百合未授粉成熟雌蕊转录组测序研究

doi:10.11924/j.issn.1000-6850.2014-0361

中国农学通报 ›› 2014, Vol. 30 ›› Issue (22): 148-154.doi: 10.11924/j.issn.1000-6850.2014-0361

所属专题：生物技术

百合未授粉成熟雌蕊转录组测序研究

蔡晓昂崔金腾贾月慧张克中

收稿日期:2014-02-18 修回日期:2014-03-11 出版日期:2014-08-05 发布日期:2014-08-05
基金资助:
北京市自然科学基金“百合卷瓣组特异种质参与的杂交育种中花粉管定向生长与胚抢救研究”

Studies on the Transcriptome Sequencing of Lilium’ s Mature Unpollinated Pistils

Received:2014-02-18 Revised:2014-03-11 Online:2014-08-05 Published:2014-08-05

摘要/Abstract

摘要： 本研究旨在大量发掘百合雌蕊特异表达基因，为研究百合杂交障碍的分子机制提供依据。利用Illumina高通量测序平台，进行百合未授粉成熟雌蕊转录组测序和数据组装，并对获得的Unigene进行功能注释、分类和代谢通路分析。“干柱头”雌蕊LoP1和“湿柱头”雌蕊LoP2分别获得40792条和39708条Unigene。2个样品的Unigene通过序列拼接，去冗余处理，以及同源转录本聚类，得到44175条All-Unigene。基于NR、NT、Swiss-Prot、GO、KEGG和COG等6个数据库进行相似性比对（E值≤10-5），依次分别有30343、21647、21281、19274、12964和22227个基因被注释。KEGG分析中，19274条AllUnigene被注释到128个代谢通路上。基因分析显示，百合未授粉成熟雌蕊已为授粉受精做好了物质、能量、信号转导以及抗病原等准备工作。

关键词: 对比分析, 对比分析

Abstract: The purpose of this study was to explore massive lilium’s pistil specially expressed genes and offer supports for explaining the molecular mechanism of lilium’s cross barriers. Based on the Illumina highthrough sequencing platform, the transcriptome sequencing of Lilium’s mature unpollinated pistils was conducted, the dates were assembled and the metabolism pathways were analyzed. 40792 and 39708 Unigene were disclosed in the“dry stigma”pistils LoP1 and the“wet stigma”pistils LoP2 respectively. A total of 44175 All-Unigene were generated by means of assembling sequence, removing the redundancy and clustering homologous transcripts between the two samples. 30343, 21647, 21281, 19274, 12964 and 22227 Unigene were respectively matched to the NR, NT, Swiss-Prot, GO, KEGG and COG databases via similarity alignment (E- value≤10- 5). By KEGG analysis, 19274 All- Unigene were annotated in the 128 metabolism pathways. Based on the genes analysis, it was indicated that something such as substance, energy, signal transduction and pathogen resistance had been ready for pollination and fertilization process in the mature unpollinated pistils.

蔡晓昂崔金腾贾月慧张克中. 百合未授粉成熟雌蕊转录组测序研究[J]. 中国农学通报, 2014, 30(22): 148-154.

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百合未授粉成熟雌蕊转录组测序研究

Studies on the Transcriptome Sequencing of Lilium’ s Mature Unpollinated Pistils

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