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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (33): 225-229.doi: 10.11924/j.issn.1000-6850.2014-0600

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

土壤硝化强度测定方法的改进

史云峰,张丽莉,赵牧秋,王刚   

  1. 琼州学院生物科学与技术学院,中国科学院沈阳应用生态研究所,琼州学院生物科学与技术学院,海南省琼州学院
  • 收稿日期:2014-03-07 修回日期:2014-11-16 接受日期:2014-04-28 出版日期:2015-01-08 发布日期:2015-01-08
  • 通讯作者: 史云峰
  • 基金资助:
    海南省自然科学基金项目“植物源生化抑制剂筛选及对土壤氮转化调控研究”(312100)；国家科技支撑计划项目“稳定性肥料关键技术研 究及产业化”(2011BAD11B04)；三亚市配套项目“植物源生化抑制剂对土壤氮转化的调控作用研究”(2013PT22)。

Improved Method of Measuring Soil Nitrification Activities

  • Received:2014-03-07 Revised:2014-11-16 Accepted:2014-04-28 Online:2015-01-08 Published:2015-01-08

摘要： 为了使土壤硝化强度的测定条件更加统一，结果更加准确，本研究针对土壤硝化强度测定时广泛采用的悬液法，研究了其最佳培养条件：反应体系pH 7.5，底物NH4+-N浓度为100 mg/L，培养温度为30℃，培养时间为5 h，风干土样预培养72 h后才可进行硝化强度的测定。还针对NO3--N生成法的不足，探讨了通过NO2--N生成法评价硝化强度的途径：通过向反应体系中添加1 mol/L的氯酸钠0.2 mL，有效抑制NO2-向NO3-的氧化。不同检测方法对硝化强度测定对比的结果发现，NO2--N生成法确是评价土壤硝化强度的最佳方法。

关键词: 纯化, 纯化

Abstract: In order to unify the measurement conditions of soil nitrification activities and increase the accuracy of results, the optimum culture conditions of suspension method for measuring soil nitrification activities were improved: the pH of the reaction system was 7.5, the substrate concentration of NH4+-N was 100 mg/L, culture temperature was 30℃, culture time was 5 h, and soil nitrification activities was measured after air-dried soil sample pre-cultured for 72 h. The study also aimed at the deficiency of preparation method of NO3--N, and discussed the method of measuring soil nitrification activities by the preparation method of NO2-- N. The method was adding 1 mol/L sodium chlorate 0.2 mL to the reaction system for effective inhibition of NO2- to NO3- oxidation. By comparing different methods, the preparation method of NO2--N was the best method of measuring soil nitrification activities.