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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (30): 56-64.doi: 10.11924/j.issn.1000-6850.2014-1242

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

棘孢木霉ACCC30536葡聚糖酶Glu41基因克隆及分析

郭瑞婷   

  1. 东北林业大学林学院森林保护学科
  • 收稿日期:2014-04-28 修回日期:2014-04-28 接受日期:2014-09-02 出版日期:2014-10-31 发布日期:2014-10-31
  • 通讯作者: 郭瑞婷
  • 基金资助:
    本科创新项目（编号：201310225005）；东北林业大学青年拔尖人才支持计划（编号：PYTT-1213-11）；国家自然科学基金面上项目（编号 31170601）

Cloning and Sequence Analysis of Glucanase Gene Glu41 from Trichoderma asperellum ACCC30536

  • Received:2014-04-28 Revised:2014-04-28 Accepted:2014-09-02 Online:2014-10-31 Published:2014-10-31

摘要： 克隆获得生物防治菌棘孢木霉（T. asperellum）ACCC30536 菌株葡聚糖酶基因Glu41 的cDNA序列和DNA序列，cDNA和DNA的GenBank接受号分别为KJ541741 和KJ541742，编码区长度为1134bp，编码378 个氨基酸。克隆获得的启动子序列长度1500bp，含有6 个逆境响应元件。BlastP 相似性分析表明该基因与Glyco-hydrolase-16 家族中棘孢木霉CBS433.97 基因同源性最高相似度是60%，SignalP 信号肽分析显示N端第25 个和第26 个氨基酸中间存在一个信号肽剪切位点（AFA||AP）。Pfam 蛋白家族分析显示它属于Glyco-hydrolase-16 家族的葡聚糖酶基因。将棘孢木霉ACCC30536 菌株葡聚糖酶基因Glu41 基因对Glyco-hydrolase-16 家族中木霉属的CBS433.97 基因组进行同源性搜索，在基因组上获得4个同源序列。4 个同源基因表达中仅有Glu41 在杨树根茎叶粉的诱导条件下表达。表明棘孢木霉葡聚糖酶基因Glu41 与生物防治相关。

关键词: 主成分分析, 主成分分析

Abstract: Glucanase gene Glu41 was cloned from the Trichoderma asperellum ACCC30536. The cDNA sequence of Glu41 is 1134 bp in length, encoding 378 amino acids. GenBank accession numbers of cDNA and DNA were KJ541741 and KJ541742, respectively. The promoter sequence was 1500bp in length and contained six stress response elements. BlastP search indicated that glucanase shared the highest similarity 60% with Glyco-hydrolase-16 superfamily of T. atroviride IMI 206040. At the same time, signalP prediction showed that the Glu41 amino acid sequence was cleaved by signal peptidase between positions 25 and 26 (AFA||AP). Pfam protein family prediction showed that Glu41 protein belonged to Glyco-hydrolase-16 superfamily. To obtain similarity gene sequences of glucanase Glu41 from the genome databases of T. asperellum CBS433.97, the amino acids sequences of Glu41 from T. asperellum ACCC30536 was used as query, resulting that 4 glucanase sequences with high similarity were obtained. Only Glu41 of those similarity genes expressed in the condition of inducing mixed powder of poplar root, stem and leaf. Above all, the study proved the glucanase gene Glu41 of T. asperellum was associated with plant disease biological control.