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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (8): 93-96.doi: 10.11924/j.issn.1000-6850.2014-2094

所属专题： 水产渔业

• 生物技术科学 • 上一篇    下一篇

一种海洋细菌显色分离培养基优化

汪 波,王伟达,李沛钿,杜 晨,颜亨梅   

  1. (北京师范大学珠海分校,广东珠海 519087）
  • 收稿日期:2014-07-31 修回日期:2014-10-24 接受日期:2014-10-29 出版日期:2015-04-07 发布日期:2015-04-07
  • 通讯作者: 汪波

Optimization of A Marine Bacterium Chromogenic Medium

(Beijing Normal University, Zhuhai Campus, Zhuhai Guangdong 519087)   

  • Received:2014-07-31 Revised:2014-10-24 Accepted:2014-10-29 Online:2015-04-07 Published:2015-04-07

摘要： 针对一种海洋细菌显色分离培养基的成分进行了探究。通过改变科玛嘉海洋细菌培养基中硫代硫酸钠(0.500、0.375、0.250、0.125 g/L)，ONPG(1.5、1.0、0.5 g/L)，纤维二糖(4.0、3.0、2.0、1.0 g/L)的浓度，观察培养基中海洋细菌菌落形态，菌落面积大小和菌落颜色，统计对比出海洋细菌在不同浓度培养基上生长的状况。结果在硫代硫酸钠浓度为0.25 g/L，纤维二糖浓度为2.0 g/L，ONPG浓度为0.5~1.0 g/L时，培养基上菌落清晰，个体适中且菌落数较多，有较好的检测效果。说明该研究在保证海洋细菌显色分离培养基分离效果前提下，降低了海洋细菌显色培养基配制成本，同时，提升了其对海洋细菌的检测效果。

关键词: 杏鲍菇, 杏鲍菇, 单因素试验, 原生质体

Abstract: A kind of marine bacteria chromogenic medium was improved to enhance effect of detection by changing the CHROMagar marine bacteria sodium thiosulfate in the culture medium (0.500; 0.375; 0.250; 0.125g/L), ONPG (1.5; 1.0; 0.5g/L), fiber two sugar (4.00; 3.0; 2.0; 1.0g/L) concentration. The results showed that there were more marine bacteria?colony numbers and more clear colony color when Sodium thiosulfate reached to 0.25g/L, Fiber two?sugar reached to 2.0g/L and ONPG reached to 0.5~1.0 g/L. It indicated that this research could reduce the cost, furthermore, it also could improve the detect effect.