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中国农学通报 ›› 2015, Vol. 31 ›› Issue (10): 101-106.doi: 10.11924/j.issn.1000-6850.2014-2607

所属专题: 食用菌 食用菌

• 林学 园艺 园林 • 上一篇    下一篇

6株金针菇菌株遗传多样性的ISSR、RAPD和SRAP综合分析

刘勇男1,常明昌2,刘靖宇2,孟俊龙2,元玲刚1,张 莉1,鲁笑笑2   

  1. (1山西农业大学园艺学院,山西太谷 030801;2山西农业大学食品科学与工程学院,山西太谷 030801)
  • 收稿日期:2014-09-26 修回日期:2015-03-16 接受日期:2014-11-13 出版日期:2015-05-05 发布日期:2015-05-05
  • 通讯作者: 常明昌
  • 基金资助:
    国家自然科学基金青年科学基金项目“基于2D LC MS/MS技术的金针菇子实体形成分子机制研究”(No. 31301826);山西省煤基重点科技攻关项目“设施食用菌高效碳循环研究与示范”(NO.FT20140301);山西农业大学科研启动基金“金针菇初生菌丝与次生菌丝的差异蛋白质组学研究”(No. xb2012019)。

Differentiation of 6 Flammulina velutipes Strains Using ISSR, RAPD and SRAP Markers

Liu Yongnan1, Chang Mingchang2, Liu Jingyu2, Meng Junlong2, Yuan Linggang1, Zhang Li1, Lu Xiaoxiao2   

  1. (1College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi 030801; 2College of Food Science and Engineering, Shanxi Agricultural University, Taigu Shanxi 030801)
  • Received:2014-09-26 Revised:2015-03-16 Accepted:2014-11-13 Online:2015-05-05 Published:2015-05-05

摘要: 为了更加快速准确的分析金针菇种质资源的遗传多样性,利用ISSR、RAPD和SRAP分子标记技术对6株金针菇菌株进行综合遗传多样性分析,并构建UPMGA亲缘关系树状图。结果表明,6株金针菇菌株间的遗传相似系数范围为0.49~0.85,在相似系数为0.59时6个菌株可分为3组,黄色菌株‘三明1号’、‘F951’、‘杂19’和白色菌株8801聚成一组,白色菌株8903和白色菌株‘金21’各成一组。该试验结果表明,3类分子标记综合分析结果相比单一分子标记分析结果能够更真实全面地体现出供试菌株之间的亲缘关系,因此该方法将有利于金针菇种质资源的评价和鉴定研究。

关键词: 抗氧化能力, 抗氧化能力

Abstract: In order to accurately analysis genetic similarity of Flammulina velutipes germplasm resources, ISSR, RAPD and SRAP molecular markers were used to differentiate 6 Flammulina velutipes strains. A dendrogram, constructed using similarity coefficients ranging from 0.49 to 0.85 clustered the 6 strains into 3 groups at a similarity coefficient of 0.59. One of them consisted of 8903, another Jin-21, and the third group the remainders. Our data demonstrated that the simultaneous use of 3 different types of markers based on different principles represented a powerful tool for analyzing genetic relationships among Flammulina velutipes strains. This method is advantageous to the identification and evaluation of Flammulina velutipes germplasm resources.