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中国农学通报 ›› 2014, Vol. 30 ›› Issue (25): 91-96.doi: 10.11924/j.issn.1000-6850.2014-0808

所属专题: 生物技术 园艺 农业气象

• 林学 园艺 园林 • 上一篇    下一篇

外源水杨酸对干旱胁迫下花椰菜叶片抗氧化作用的影响

王丽君 郁继华 张国斌 周亚婷 周德霞 杨伟 杨海兴   

  • 收稿日期:2014-03-24 修回日期:2014-04-10 出版日期:2014-09-05 发布日期:2014-09-05
  • 基金资助:
    国家现代农业产业技术体系建设资金项目“国家大宗蔬菜产业体系专项”(CARS-25-C-07);国家自然科学基金项目“铵态氮对高寒地区娃娃菜根系生理和氮素效率的调控机理研究”(31260473)。

Effect of Exogenous Salicylic Acid on Antioxidant Capacity in Cauliflower Leaves Under Drought Stress

  • Received:2014-03-24 Revised:2014-04-10 Online:2014-09-05 Published:2014-09-05

摘要: 以花椰菜为试材,采用大田人工控水设置田间持水量的35%、50%和65% 3个灌水下限,通过叶面喷施0.1 mmol/L水杨酸(SA),研究外源SA对花椰菜叶片抗氧化能力的影响。结果表明:SA可提高叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)等抗氧化酶活性,同时减缓花椰菜叶片中丙二醛(MDA)积累和细胞膜透性的持续增大,增加游离脯氨酸(Pro)和还原型谷胱甘肽(GSH)在叶片中的积累。因此,SA能够提高干旱胁迫下花椰菜的抗旱能力,提高花椰菜叶片中抗氧化物质的活性,增加渗透调节物质的含量,减少膜脂过氧化作用,在一定程度上可缓解干旱对植物造成的伤害。

关键词: 遗传多样性, 遗传多样性

Abstract: The experiment was conducted with cauliflower to study the influence of exogenous salicylic acid (SA) on oxidation resistance of cauliflower in the field, which set three lower irrigation limits of water control, that was 35%, 50%, 65% of field capacity. The result showed that SA could increase the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbic acid peroxidase (APX). Meanwhile, SA reduced accumulation of cauliflower leaves malondialdehyde (MDA) content and continuously increased cell membrane permeability, increased accumulation of free proline (Pro) and glutathione (GSH) in cauliflower leaves. Therefore, under drought stress, SA could improve the drought resistance ability and antioxidant activity, increased content of osmoregulation substance of cauliflower, reduced lipid peroxidation of membrane. To some extent, SA could alleviate the drought damage on plant.