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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (12): 55-60.doi: 10.11924/j.issn.1000-6850.casb15090096

• 农学 农业基础科学 • 上一篇    下一篇

低温胁迫下三七一年生紫、绿地上茎植株抗氧化能力的研究

孙 艳1,赵昶灵1,2,余育启3,陈中坚3,文国松1,2,唐小华1,魏富刚3,肖兴磊3,李孙文4   

  1. (1云南农业大学农学与生物技术学院,昆明 650201；2云南农业大学特色资源植物改良与利用研究所,昆明 650201；3文山市苗乡三七实业有限公司,云南文山 663000；4云南农业大学农科专业基础实验教学中心,昆明 650201）
  • 收稿日期:2015-09-23 修回日期:2016-04-11 接受日期:2016-03-04 出版日期:2016-04-27 发布日期:2016-04-27
  • 通讯作者: 赵昶灵
  • 基金资助:
    国家自然科学基金项目“三七花色苷对三七的生态生理作用”(31260091)，“一年生三七地上茎的黄金分割式紫化及其与皂苷积累的关系”(31460065)。

Antioxidant Capacity of Purple and Green Aerial Stem Plants of One-year-old Panax notoginsengUnder Low Temperature Stress Simulated by Icy Water

Sun Yan1, Zhao Changling1,2, Yu Yuqi3, Chen Zhongjian3, Wen Guosong1,2, Tang Xiaohua1,Wei Fugang3, Xiao Xinglei3, Li Sunwen4   

  1. (1College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201;2Institute of the Improvement and Utilization of Characteristic Resource Plants, Yunnan Agricultural University, Kunming 650201; 3Miaoxiang Sanqi Industrial Corporation Ltd. of Wenshan City, Wenshan Yunnan 663000; 4Teaching Center of the Basic Experiments of Agricultural Majors, Yunnan Agricultural University, Kunming 650201)
  • Received:2015-09-23 Revised:2016-04-11 Accepted:2016-03-04 Online:2016-04-27 Published:2016-04-27

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摘要： 旨在探究三七地上茎积累花色苷对其抗寒性的效应。研究了冰水模拟低温胁迫下三七一年生紫、绿地上茎植株叶片可溶性蛋白质和丙二醛(MDA)含量及抗氧化酶比活力。结果表明，在冰水模拟低温胁迫下，紫、绿地上茎植株叶片的可溶性蛋白质含量和过氧化氢酶(CAT)比活力及绿地上茎植株叶片的MDA含量均上升，紫、绿茎植株叶片的过氧化物酶(POD)和超氧化物歧化酶(SOD)比活力及紫茎植株叶片的MDA含量均下降，且紫茎叶片可溶性蛋白质含量和CAT比活力的升幅及绿茎叶片POD和SOD比活力的降幅均更大；在冰水处理结束时，紫茎植株叶片的可溶性蛋白质含量、POD和SOD的比活力均高于绿茎的，但CAT比活力和MDA含量略低于绿茎的。但是，紫、绿茎植株叶片的可溶性蛋白质和MDA含量及抗氧化酶比活力的差异均未达到显著水平。因此，三七地上茎积累花色苷利于其抗寒。

关键词: 叶用莴苣, 叶用莴苣, 目标区域扩增多态性(TRAP), PCR, 体系优化

Abstract: The aim was to explore the effects of anthocyanin accumulation in the aerial stems of Panax notoginseng on the cold resistance of P. notoginseng. The soluble protein and malondialdehyde (MDA) contents and the antioxidase specific activities in the leaves of one-year-old P. notoginseng plants with purple and green aerial stems under the low temperature stress simulated by icy water were studied. The results showed that, under the low temperature stress simulated by icy water, the soluble protein contents and the specific activities of the catalase (CAT) in the leaves of one-year-old P. notoginseng plants with purple and green aerial stems, and the MDA contents in the leaves of the green aerial stem plants all increased, the specific activities of the peroxidase (POD) and superoxide dismutase (SOD) in the leaves of the purple and green aerial stem plants, and the MDA contents in the leaves of the purple aerial stem plants all decreased. Moreover, the increasing degrees of the soluble protein contents and CAT specific activities in the leaves of the purple aerial stem plants and the decreasing degrees of the specific activities of POD and SOD in the leaves of the green aerial stem plants were both much larger. When the icy water treatment terminated, the soluble protein content, and the specific activities of the POD and SOD in the leaves of the purple aerial stem plants were higher than those of the green aerial stem plants, whereas the CAT specific activity and MDA content in the leaves of the purple aerial stem plants were slightly lower than those of the green aerial stem plants. However, the differences of the contents of soluble protein and MDA and the specific activities of three antioxidases of the purple and green aerial stem plants did not reach the significant level. Thus, the anthocyanin accumulation in the aerial stems of P. notoginseng was good for the cold resistance of P. notoginseng.

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