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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (23): 89-94.doi: 10.11924/j.issn.1000-6850.casb15030036

所属专题： 生物技术

• 植物保护 农药 • 上一篇    下一篇

榕管蓟马防治高效菌株蜡蚧轮枝菌V3450固态发酵基质配方筛选

余德亿1,姚锦爱1,黄 鹏1,董金龙2   

  1. (1福建省农业科学院植物保护研究所,福州 350013；2长泰金诺农业科技有限公司,福建漳州 363204）
  • 收稿日期:2015-03-05 修回日期:2015-03-16 接受日期:2015-03-17 出版日期:2015-08-20 发布日期:2015-08-20
  • 通讯作者: 余德亿
  • 基金资助:
    福建省农业科技重大专项专题“花果主要病虫害检测及生态调控产品研制与示范”(2013NZ0002-4-7)；福建省属公益优势领域重点项目“设施花果蓟马生态防控技术研究与示范”(2014R1024-1)；福建省自然科学基金“榕管蓟马与蜡蚧轮枝菌间的互作关系及致害机理研究”(2014J01110)；福建省农业科学院科技创新团队项目“捕食螨携带虫生真菌防治害虫的产品研制与应用”(CXTD-2-1313)。

Selection of the Solid-state Fermentation Media Formula of Lecanicillium lecanii V3450 High Efficient Strain to Control Gynaikothrips uzeli

Yu Deyi1, Yao Jin’ai1, Huang Peng1, Dong Jinlong2   

  1. (1Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013;2Changtai Kino Agricultural Science and Technology Limited Company, Zhangzhou Fujian 363204)
  • Received:2015-03-05 Revised:2015-03-16 Accepted:2015-03-17 Online:2015-08-20 Published:2015-08-20

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摘要： 蜡蚧轮枝菌V3450是榕管蓟马防治的高效菌株，为实现该菌株的放大培养，以盆栽榕树出口使用的主要栽培基质椰糠和单一固态发酵培养产孢量最多的麦麸为主载体，开展该菌株的固态发酵基质一般组合配方和正交组合配方筛选试验。结果表明：一般组合固态发酵基质配方5（椰糠+麦麸+磷酸盐+酵母粉=7.5 g+7.5 g+0.2 g+0.5 g）为最好的初筛配方，蜡蚧轮枝菌V3450菌株固态发酵培养所需基质的4个基本组分因子为麦麸、椰糠、磷酸盐和酵母粉；正交组合固态发酵基质配方8（麦麸:椰糠:磷酸盐:酵母粉=3:2:3:2）为最佳的组合配方，麦麸、椰糠、磷酸盐和酵母粉等4个基本组分因子的最佳质量配比为3:2:3:2。正交组合配方8（麦麸:椰糠:磷酸盐:酵母粉=3:2:3:2）是蜡蚧轮枝菌V3450菌株的最佳固态发酵基质配方，放大培养的真菌孢子对榕管蓟马成虫具有很强的侵染力。

关键词: 遗传标记, 遗传标记, 核桃, 遗传多样性

Abstract: The Lecanicillium lecanii V3450 was a high efficient strain to control Gynaikothrips uzeli. In order to realize target of amplification culture, the author used export potted ficus with the main growing media coco coir and the most sporulation quantity in single solid-state fermentation media wheat bran as the main carrier, to select a solid-state fermentation media of Lecanicillium lecanii V3450 strain through the general and orthogonal combination design experiment. The results showed that the best media formula of preliminary selection in the general combination solid-state fermentation media was number 5 (coir dust wheat bran phosphate yeast powder =7.5 g 7.5 g 0.2 g 0.5 g), the four basic component factors in the solid-state fermentation media formula of Lecanicillium lecanii V3450 strain were wheat bran, coco coir, phosphate and yeast powder. The optimal media formula of careful selection in the orthogonal combination solid-state fermentation media was number 8 (coir dust : wheat bran : phosphate : yeast powder =3:2:3:2), the optimal mass ratio of four basic component factors including wheat bran, coco coir, phosphate and yeast powder was 3:2:3:2. The optimal solid-state fermentation media formula of Lecanicillium lecanii V3450 strain was orthogonal combination formula 8 (coir dust : wheat bran : phosphate : yeast powder =3:2:3:2), and its fungal spores had very strong infectivity to Gynaikothrips uzeli adults.