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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (25): 100-106.doi: 10.11924/j.issn.1000-6850.casb15030080

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

桃芽休眠诱导过程中IAA、ABA 含量及PpPINs 基因表达的变化

贾 楠,田 航,彭竹君,王 忆,吴 婷,张新忠   

  1. 中国农业大学农学与生物技术学院,北京品友互动信息技术有限公司,上海晶纯生化科技股份有限公司,中国农业大学农学与生物技术学院园艺植物研究所,中国农业大学农学与生物技术学院园艺植物研究所,中国农业大学农学与生物技术学院园艺植物研究所
  • 收稿日期:2015-03-11 修回日期:2015-07-23 接受日期:2015-03-27 出版日期:2015-09-23 发布日期:2015-09-23
  • 通讯作者: 张新忠
  • 基金资助:
    国家支撑课题“设施果树和花卉优质高效低耗生产关键技术研究与示范”(2011BAD12B02)。

Changes in IAA and ABA Levels and PpPINs Genes Expression During the Bud Dormancy Induction in Peach

  • Received:2015-03-11 Revised:2015-07-23 Accepted:2015-03-27 Online:2015-09-23 Published:2015-09-23

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摘要： 为认识桃芽休眠诱导过程中生理与相关基因表达的变化，分别用HPLC检测‘中农红久保’、‘中农3 号’、03-30-035 3 个桃品种（系）休眠诱导时期叶片和叶芽IAA和ABA含量，用RT-qPCR检测叶片和叶芽生长素极性运输载体基因PpPIN1、PpPIN3、PpPIN5 和PpPIN8 共4 个基因的相对表达量。结果表明，在桃芽休眠诱导过程中，叶片IAA含量未见显著变化，ABA含量自10 月6 日开始升高，至10 月16 日含量达到高峰；叶芽中10 月上中旬IAA 含量骤增，而ABA含量逐渐降低。本研究中桃基因组的4 个PpPINs 基因家族成员中，叶片中活跃表达为PpPIN5，其次为PpPIN3，在10 月上中旬PpPIN5 出现强烈表达；而在10 月26 日，叶芽中活跃表达的PpPIN1 和PpPIN3 表达量骤降。桃休眠诱导过程中叶片和叶芽内内源激素和PpPINs基因表达量变化明显不同。

关键词: 橡胶树, 橡胶树, 砧木, 接穗, 产量, 养分含量

Abstract: This study focused on the changes of physiology and relative gene expression during the bud dormancy induction in peach. The contents of IAA and ABA were measured by HPLC and the relative genes expression of auxin polar transport carrier genes PpPIN1, PpPIN3, PpPIN5 and PpPIN8 was detected by RTqPCR in leaves and vegetative buds. Three peach varieties,‘Zhongnonghongjiubao’,‘Zhongnongsanhao’, 03- 30-035, were chosen as the test materials. Results showed that during the bud dormancy induction in peach, the content of IAA did not show a significant change, but the content of ABA rose since October 6 and then reached the peak on October 16. Nevertheless, the content of IAA surged in leaf buds while the content of ABA decreased gradually. Among the four genes mentioned above, PpPIN5, followed by PpPIN3, was in active expression in leaves and had a strong expression in the early to mid-October. However, the relative expression of PpPIN1 and PpPIN3 dropped suddenly in October 26 in vegetative buds. In general, the changes of endogenous hormones and PpPINs genes relative expression were significantly different in leaves and vegetative buds during the bud dormancy induction in peach.