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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (26): 12-17.doi: 10.11924/j.issn.1000-6850.casb15040074

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

外源维生素对高NaCl胁迫下紫花苜蓿种子萌发及恢复性的影响

刘艳军,范 晶,韩学珅,李 斌,张 义   

  1. (黑龙江大学农业资源与环境学院/黑龙江大学农业资源利用与环境安全重点实验室,哈尔滨 150080）
  • 收稿日期:2015-04-09 修回日期:2015-08-08 接受日期:2015-06-23 出版日期:2015-09-23 发布日期:2015-09-23
  • 通讯作者: 张 义
  • 基金资助:
    黑龙江大学博士启动基金项目“两型豆knox-I、lfy基因克隆与表达分析”(2006-08)；黑龙江大学实验室开放基金项目“外源VPP和VB6对NaCl胁迫下紫花苜蓿种子萌发特性的影响”(14K213)；创新训练项目“VC和VB1对NaCl胁迫下紫花苜蓿种子萌发特性的影响”(2014NY14)。

Effects of Exogenous Vitamins on Seed Germination and Recovery of Medicago sativa L. Under High NaCl Stress

Liu Yanjun, Fan Jing, Han Xueshen, Li Bin, Zhang Yi   

  1. (Key Laboratory of Agricultural Resources Utilization and Environmental Security of Heilongjiang University/College of Agricultural Resources and Environment, Heilongjiang University, Harbin 150080)
  • Received:2015-04-09 Revised:2015-08-08 Accepted:2015-06-23 Online:2015-09-23 Published:2015-09-23

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摘要： 为给改进紫花苜蓿在盐碱地区的生产提供依据，笔者研究了14610 mg/L高浓度NaCl胁迫下，外源水溶性维生素（VB1、VB2、VPP、VB6、VH、VB12与VC）对紫花苜蓿种子萌发及恢复萌发性状的影响。结果表明：62.5 mg/L VPP显著提高了恢复萌发后的芽鲜重，1000 mg/L VB6、500 mg/L VB12和1000 mg/L VC促进了紫花苜蓿高盐胁迫下的萌发，但对胁迫减轻后的恢复萌发没有显著影响。250 mg/L VB12对高盐胁迫下紫花苜蓿的萌发及恢复萌发均有显著性改进，使高NaCl的致死性从19.667%降低到4.667%。VB1、VB2和VH未能有效改进高NaCl胁迫对萌发的抑制效应。试验显示外源VB6、VB12、VC对高NaCl胁迫下紫花苜蓿种子的萌发特性有一定程度的调节作用。

关键词: 高光谱, 高光谱, 水稻土, 有机质, 多元逐步回归, 偏最小二乘回归, 估算

Abstract: In order to provide basis for the production of Medicago sativa L. in saline-alkali soil, the author assessed the effect of exogenous water-soluble vitamins (VB1, VB2, VPP, VB6, VH, VB12 and VC) on the seed germination and recovery of M. sativa under 14610 mg/L NaCl stress. The results showed that 62.5 mg/L VPP significantly increased the shoot fresh weight after the recovery germination. 1000 mg/L VB6, 500 mg/L VB12 and 1000 mg/L VC substantially promoted the germination under high salinity stress, but had no significant impact on the recovery germination after the stress was relieved. 250 mg/L VB12 significantly improved both the germination under high NaCl stress and the recovery germination, reduced the mortality rate under high NaCl stress from 19.667% to 4.667%. VB1, VB2 and VH failed to effectively alleviate the inhibitory effect of high NaCl stress on seed germination. The experiment showed that exogenous VB6, VB12 and VC could play a role in regulating the seed germination characteristic of M. sativa under high NaCl stress.