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中国农学通报 ›› 2015, Vol. 31 ›› Issue (27): 198-202.doi: 10.11924/j.issn.1000-6850.casb15050021

所属专题: 生物技术 园艺

• 生物技术科学 • 上一篇    下一篇

甜菜保护酶系统对硼胁迫响应及其与内源激素相关性研究

宋柏权,刘乃新,吴玉梅,闫志山,裴轩瑗,杨 骥   

  1. 黑龙江大学农作物研究院,黑龙江大学农作物研究院,黑龙江大学农作物研究院,黑龙江大学农作物研究院,哈尔滨工业大学,黑龙江大学农作物研究院
  • 收稿日期:2015-05-06 修回日期:2015-06-12 接受日期:2015-08-21 出版日期:2015-09-23 发布日期:2015-09-23
  • 通讯作者: 宋柏权
  • 基金资助:
    国家农产品质量安全风险评估专项(GJFP2015011);现代农业产业技术体系建设专项基金“东北区甜菜栽培”(CARS210301-03);黑龙江省普通高校遗传育种实验室开放课题。

Effects of Boron Stress on Protective Enzyme System of Sugar Beet and Its Relationship with Endogenous Hormones

刘乃新,吴玉梅,,裴轩瑗 and   

  • Received:2015-05-06 Revised:2015-06-12 Accepted:2015-08-21 Online:2015-09-23 Published:2015-09-23

摘要: 研究不同硼素供应水平下, 硼对甜菜保护酶系统的影响规律以及保护酶系统与内源激素相关性, 丰富甜菜生理学理论。以甜菜单粒种 ‘HI0099’ 为试验材料, 在水培条件下, 设置 4个硼营养浓度, 对甜菜叶片保护酶系统以及内源激素含量进行测定。结果表明: 4种不同硼素处理的甜菜, 叶片中丙二醛(MDA)含量均随着生育进程有减少趋势, 而脯氨酸含量均呈增加趋势; 硼素胁迫处理时, 叶片 MDA含量以及脯氨酸含量增加。不同硼素处理下, 甜菜叶片超氧化物歧化酶(SOD)活性、 过氧化物酶(POD)活性表现为随生育进程逐渐升高趋势; 同一日期, 不同硼浓度表现为缺乏或者毒害胁迫下 SOD活性有升高的趋势, 并且随着甜菜生长, 硼胁迫情况下 SOD活性处理间差异有变小趋势; 在硼胁迫条件下, SOD活性与 ZT含量呈显著负相关(P<0.05), 与 GA3呈极显著负相关(P<0.01)。不同日期各硼素营养处理间过氧化氢酶(CAT)活性变化规律一致, 呈现先降低后升高的趋势。综合分析说明, 硼素胁迫引起膜脂过氧化, 甜菜叶片通过提高保护酶活性来防御硼毒害, 但其防御功能是有限的, 随着生育进程, 甜菜对硼胁迫防御功能达到临界值, 保护酶活性变化不再显著, 且甜菜植株在硼素胁迫下的生长发育受保护酶系统及内源激素间共同调控。

关键词: 东北刺人参, 东北刺人参, 不定根, 生物反应器, 增殖

Abstract: In order to study the effect of boron on sugar beet protective enzyme system and the correlation between protective enzyme system and endogenous hormones under different boron levels, and then enrich the physiological theory of sugar beet, sugar beet‘HI0099’was selected as experimental material. Under hydroponic condition, four nutrition concentrations of boron were set to determine the protective enzyme system of sugar beet leaves and endogenous hormones content. The results showed that, for the four varieties of sugar beet under different boron treatments, MDA content of leaves decreased and proline content increased as the plant growth. MDA content of leaves and proline increased under boron stress treatments. Under different boron treatments, SOD activity of sugar beet leaves and POD increased as plant growth. In the same date, different boron concentrations presented a trend of deficiency or the SOD activity increased under toxicity stress. Under boron stress condition, SOD activity and ZT content presented a significantly negative correlation (P<0.05); SOD activity and GA3 presented a highly and significantly negative correlation (P<0.01). In different dates, the variation rule of CAT activity among all the boron nutrition treatments was the same which firstly decreased and then increased. Comprehensive analysis showed that boron stress caused membrane lipid peroxidation. Sugar beet leaves defended boron toxicity by increasing protective enzyme activity; however, its defensive capability was limited. With the plant growing, the defensive capability of sugar beet on boron stress reached a critical value. The variation of protective enzyme activity was no longer significant. Sugar beet plant growth which was under boron stress condition was controlled by protective enzyme system and endogenous hormones.