污泥高温堆肥过程中细菌群落动态特征

doi:10.11924/j.issn.1000-6850.casb15060130

中国农学通报 ›› 2015, Vol. 31 ›› Issue (35): 164-171.doi: 10.11924/j.issn.1000-6850.casb15060130

• 资源环境生态土壤气象 • 上一篇下一篇

污泥高温堆肥过程中细菌群落动态特征

刘建斌,熊建军,张淑彬,刘培财,殷晓芳,王幼珊

北京市农林科学院植物营养与资源研究所,北京城市排水集团有限责任公司,北京市农林科学院植物营养与资源研究所,北京城市排水集团有限责任公司,北京市农林科学院植物营养与资源研究所,北京市农林科学院植物营养与资源研究所

收稿日期:2015-06-25 修回日期:2015-08-20 接受日期:2015-08-25 出版日期:2015-12-18 发布日期:2015-12-18
通讯作者: 王幼珊
基金资助:
“十二五”农村领域国家科技计划项目“华北集约化农区农业面源污染防控技术集成与示范”(2012BAD15B02-1)；北京市优秀人才培养资助项目“北京地区设施土壤连作条件下微生物群落响应研究”(2013D002020000004)；北京市农林科学院科技创新能力建设专项“鸡粪堆肥原位除臭工艺及厂区环境优化技术支撑研究”(KJCX20151205)。

Microbial Community Dynamics During the Sludge Thermophilic Composting Process

Received:2015-06-25 Revised:2015-08-20 Accepted:2015-08-25 Online:2015-12-18 Published:2015-12-18

摘要/Abstract

摘要： 研究旨在明确污泥高温堆肥过程中细菌微生物群落的动态变化规律，为城市污泥堆肥快速发酵提供理论依据和技术支持。以污泥工厂化高温好氧堆肥为研究对象，通过传统平板计数法、变性梯度凝胶电泳(DGGE)和高通量测序技术对污泥高温堆肥过程中的主要细菌组成和变化趋势进行了分析。传统平板计数法结果表明，在污泥堆肥过程中，常温细菌(28℃)数量表现为先降后升的变化趋势，而高温细菌(55℃)的数量表现为先升后降的变化趋势。在不同的堆肥过程中共分离到6 株常温细菌（Paracoccus thiocyanatus，Advenella sp.，Arthrobacter sp.，Lysobacter sp.，Serratia sp. 和Rhodococcus pyridinivorans）和2 株高温细菌（Ureibacillus thermosphaericus 和Geobacillus denitrificans）。DGGE的图谱结果显示，堆肥过程中细菌的组成有明显的变化，聚类分析表明微生物类群分属4 个不同类群。污泥堆肥高温期（堆肥第7 天）细菌高通量测序结果显示，主要细菌分布在6 个门，8 个纲中，其中88.6%的细菌属于Chloroflexi 门，Thermomicrobia 纲，Sphaerobacte 目中的细菌。在城市污泥工厂化堆肥过程中细菌组成和数量处于明显的变化过程中，Sphaerobacte 目中的细菌是污泥堆肥高温阶段的优势微生物。

关键词: 卷丹, 卷丹, 茎尖, 继代次数, 超低温保存, 存活率, 显微结构

Abstract: The aims of this study was to illustrate the dynamic changes of bacterial community during the process of municipal sludge composting, and provided theoretical basis and technical support to accelerate the compost process. The bacterial community structures and dynamic changes were analyzed by the culture-dependent method (plate count method) and culture-independent methods (denaturing gradient gel electrophoresis, and high throughput sequencing) during the municipal sludge composting process. The mesophilic bacteria (28℃) were firstly decreased and got increased gradually with the compost process. Instead, thermophilic bacteria (55℃) were declined after stimulation at the beginning of compost. Six mesophilic bacteria (Paracoccus thiocyanatus, Advenella sp., Arthrobacter sp., Lysobacter sp., Serratia sp. and Rhodococcus pyridinivorans) and 2 thermophilic bacteira (Ureibacillus thermosphaericus and Geobacillus thermodenitrificans) were isolated by the culture-dependent method. During the composting process, bacteria diversity changed obviously resulted by the DGGE fingerprint and 4 branches were divided by the cluster analysis. There were 6 phyla, 8 classes of the predominant bacteria were gained by the high throughput sequencing (the 7th day of the composting), and 88.6% of the bacteria distributed in Sphaerobacte order, Thermomicrobia class, Chloroflexi phylum. The results of this study revealed that bacteria belonged to Sphaerobacte order were predominant in the amount and diversity in the composting.

中图分类号:

S141.4

刘建斌,熊建军,张淑彬,刘培财,殷晓芳,王幼珊. 污泥高温堆肥过程中细菌群落动态特征[J]. 中国农学通报, 2015, 31(35): 164-171.

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污泥高温堆肥过程中细菌群落动态特征

Microbial Community Dynamics During the Sludge Thermophilic Composting Process

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