欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2020, Vol. 36 ›› Issue (21): 113-117.doi: 10.11924/j.issn.1000-6850.casb20190600271

所属专题： 马铃薯

• 生物科学 • 上一篇    下一篇

不同浓度NAA/6BA配比对不同品种甘薯茎尖培养特性的影响

唐丽雪1, 杜进辉1, 史春余1(), 柳洪鹃1, 孟迪1, 刘化学2, 闫锴1, 康新远1   

  1. 1 山东农业大学农学院,山东泰安 271018
    2 汶上县地瓜研究所,山东汶上 272500
  • 收稿日期:2019-06-11 修回日期:2019-11-13 出版日期:2020-07-25 发布日期:2020-07-21
  • 通讯作者: 史春余
  • 作者简介:唐丽雪,女,1995年出生,山东寿光人,在读硕士,研究方向：作物高产优质生理生态。通信地址：271018 山东省泰安市岱宗大街61号 山东农业大学农学院甘薯实验室,E-mail：15244346076@163.com。
  • 基金资助:
    山东省薯类产业创新团队“首席专家”项目(SDAIT-16-01);济宁市重点研发计划项目“甘薯脱毒苗工厂化快速繁育关键技术研究”(2018NYNSO14)

NAA/6BA Ratio at Different Concentrations: Effects on Shoot Tip Culture Characteristics of Different Sweet Potato Varieties

Tang Lixue1, Du Jinhui1, Shi Chunyu1(), Liu Hongjuan1, Meng Di1, Liu Huaxue2, Yan Kai1, Kang Xinyuan1   

  1. 1 College of Agronomy, Shandong Agricultural University, Tai’an Shandong 271018
    2 Wenshang Institute of Sweet Potato, Wenshang Shandong 272500
  • Received:2019-06-11 Revised:2019-11-13 Online:2020-07-25 Published:2020-07-21
  • Contact: Shi Chunyu

RichHTML

3

PDF (PC)

41

摘要：

为明确不同品种甘薯茎尖培养的最佳NAA/6BA配比,设置0.1 mg/L/2.5 mg/L、0.2 mg/L/2.5 mg/L、0.2 mg/L/1.0 mg/L 3组NAA/6BA浓度配比处理,观测不同浓度激素处理对‘北京553’、‘红香蕉’、‘苏薯8号’、‘烟薯25’、‘安吉芋’、‘济徐23’、‘渝紫7号’、‘商薯19’茎尖培养成苗率、愈伤组织直径、不定根数目、叶片数和植株高度的影响,同时调查不同品种试管苗的移栽成活率。结果表明,‘红香蕉’、‘济徐23’、‘渝紫7号’、‘商薯19’在0.1 mg/L/2.5 mg/L的处理下培养效率最高;‘安吉芋’在0.2 mg/L/2.5 mg/L的处理下培养效率最高;‘苏薯8号’、‘北京553’、‘烟薯25’在0.2 mg/L/1.0 mg/L的处理下培养效率最高。因此,在甘薯茎尖培养过程中,不同甘薯品种适宜的NAA/6BA配比存在显著差异,在实际生产中应根据品种特性来进行调整激素的用量和比例。

关键词: 甘薯, NAA/6BA配比, 茎尖培养, 试管苗, 成苗率, 培养效率

Abstract:

To determine the best NAA/6BA ratio for shoot tip culture of different varieties of sweet potato, we set up NAA/6BA ratio at 3 concentrations (0.1 mg/L/2.5 mg/L, 0.2 mg/L/2.5 mg/L, 0.2 mg/L/1.0 mg/L), and studied the effects of different hormone treatments on the shoot tip culture seedling rate, callus diameter, adventitious root number, leaf number and plant height of ‘Beijing 553’, ‘Hongxiangjiao’, ‘Sushu 8’, ‘Yanshu 25’, ‘Anjiyu’, ‘Jixu 23’, ‘Yuzi 7’, ‘Shangshu 19’, at the same time, investigated the transplant survival rate of test-tube plantlets of different varieties. The results showed that: the culture efficiency of ‘Hongxiangjiao’, ‘Jixu 23’, ‘Yuzi 7’ and ‘Shangshu 19’ was the highest under the treatment of 0.1 mg/L/2.5 mg/L, that of ‘Anjiyu’ was the highest under the treatment of 0.2 mg/L/2.5 mg/L, and that of ‘Sushu 8’, ‘Beijing 553’ and ‘Yanshu 25’ was the highest under the treatment of 0.2 mg/L/1.0 mg/L. In the process of shoot tip culture of sweet potatoes, there are significant differences of the appropriate ratio of NAA/6BA among different varieties, and the dosage and proportion of hormones should be adjusted according to the characteristics of the varieties in practical production.

Key words: sweet potato, NAA/6BA ratio, shoot tip culture, test-tube plantlets, seedling rate, cultivation efficiency

中图分类号: