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中国农学通报

中国农学通报 ›› 2021, Vol. 37 ›› Issue (18): 25-30.doi: 10.11924/j.issn.1000-6850.casb2020-0422

所属专题： 马铃薯

• 农学·农业基础科学 • 上一篇    下一篇

MS培养基不同成分对马铃薯脱毒试管苗生长的影响

贾小霞1,2,3(), 齐恩芳1,2,3, 刘石1,2,3, 黄伟1,2,3, 吕和平1,2,3, 文国宏1,2,3, 马胜1,2,3   

  1. 1甘肃省农业科学院马铃薯研究所,兰州 730070
    2甘肃省马铃薯种质资源创新工程实验室,兰州 730070
    3农业农村部西北旱作马铃薯科学观测实验站,甘肃渭源 748201
  • 收稿日期:2020-09-04 修回日期:2020-12-16 出版日期:2021-06-25 发布日期:2021-07-13
  • 作者简介:贾小霞,女,1978年出生,甘肃定西人,副研究员,研究生,博士,主要从事马铃薯种质改良研究工作。通信地址：730070 甘肃省兰州市安宁区农科院新村1号 甘肃省农业科学院马铃薯研究所,Tel：0931-7758100,E-mail： jiaxx0601@163.com。
  • 基金资助:
    甘肃省农业科学院科技创新专项“抗草铵膦马铃薯种质早代筛选技术研究”(2020GAAS16);甘肃省农业科学院生物技术育种专项“马铃薯分子育种技术创新及高淀粉新品种选育”(2020GAAS10);国家现代农业产业技术体系“高淀粉品种改良岗位”(CARS-09-P06)

Different Components of MS Medium: Effects on the Growth of Potato Virus-free Test-tube Seedlings

Jia Xiaoxia1,2,3(), Qi Enfang1,2,3, Liu Shi1,2,3, Huang Wei1,2,3, Lv Heping1,2,3, Wen Guohong1,2,3, Ma Sheng1,2,3   

  1. 1Potato Research Institute of Gansu Academy of Agricultural Sciences, Lanzhou 730070
    2Gansu Engineering Laboratory of Potato Germplasm Resources Innovation, Lanzhou 730070
    3Scientific Observing and Experimental Station of Potato Dry Farming in Northwest China, Ministry of Agriculture and Rural Affairs, Weiyuan Gansu 748201
  • Received:2020-09-04 Revised:2020-12-16 Online:2021-06-25 Published:2021-07-13

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摘要：

为了探索马铃薯脱毒苗高效低成本快繁培养基,以‘陇薯7号’和‘陇薯20号’试管苗为试材,测定不同培养基上试管苗的株高、茎粗、叶片数、茎叶鲜干重和叶绿素含量。以除去有机的MS培养基为对照,在仅缺钙的培养基上,两品种都能正常生长,但‘陇薯20号’的株高显著低于对照;仅缺乏微量元素或同时缺乏钙盐和微量元素时,‘陇薯7号’与对照无显著差异,但‘陇薯20号’的株高显著低于对照;缺铁使‘陇薯7号’新芽失绿,生长受阻,但对‘陇薯20号’没有产生不利影响,且铁和钙盐同时缺乏有利于‘陇薯20号’的生长;在只含MS大量元素的培养基培养时,两品种前期均出现茎叶微黄的现象,但随培养时间的延长,黄色逐渐消失,且‘陇薯7号’的各指标显著高于对照。由此可见,不同品种对营养元素的需求差异较大,‘陇薯7号’和‘陇薯20号’分别可以在只含大量元素和大量元素+微量元素的MS培养基上高效快繁。

关键词: 马铃薯试管苗, MS培养基, 脱毒苗, 钙, 铁, 微量元素

Abstract:

To explore high-efficiency and low-cost media for rapid propagation of virus-free potato seedlings, ‘Longshu 7’ and ‘Longshu 20’ were used as test materials, the plant height, stem diameter, the number of leaves, fresh/dry weight of stems and leaves, and chlorophyll content of test-tube seedlings on different media were determined. The results showed that compared with MS medium with organic removal, both varieties grew normally on the medium lacking calcium, but the plant height of ‘Longshu 20’ was significantly lower than that of the control. When only lacking trace elements or lacking calcium salt and trace elements at the same time, there was no significant difference between ‘Longshu 7’ and the control, but the plant height of ‘Longshu 20’ was significantly lower than that of the control. The lack of iron made the new bud of ‘Longshu 7’ lose green and the growth was hindered, but it had no adverse effect on ‘Longshu 20’, and the lack of iron and calcium salt at the same time was beneficial to the growth of ‘Longshu 20’. When cultured on the medium containing only MS macro elements, the stems and leaves of the two varieties appeared yellowish at the early stage, but with the extension of the cultivation time, the yellowness gradually disappeared, and the indexes of ‘Longshu 7’ were significantly higher than those of the control. Therefore, different varieties have different requirements for nutrient elements, and ‘Longshu 7’ and ‘Longshu 20’ can be efficiently and rapidly propagated on MS medium containing only macro elements and macro elements + trace elements, respectively.

Key words: potato test-tube seedling, MS medium, virus-free seedling, calcium, iron, trace element

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