芽胞杆菌中的喷他霉素检测与分析

doi:10.11924/j.issn.1000-6850.casb15080088

中国农学通报 ›› 2016, Vol. 32 ›› Issue (12): 98-102.doi: 10.11924/j.issn.1000-6850.casb15080088

芽胞杆菌中的喷他霉素检测与分析

陈峥,刘波,朱育菁,潘志针,刘国红

(福建省农业科学院农业生物资源研究所,福州 350003）

收稿日期:2015-08-18 修回日期:2016-04-18 接受日期:2015-12-23 出版日期:2016-04-27 发布日期:2016-04-27
通讯作者: 刘波
基金资助:
公益性行业（农业）科研专项经费“功能性微生物制剂在农业副产物资源转化与利用中的研究与示范”(201303094)；福建省农科院院青年创新基金“芽胞杆菌种质资源库EIP信息平台网站构建”(2013dqd-6)；福建省农科院杰出青年人才基金“芽胞杆菌资源的保护与利用”(2014JQ-2)。

Detection and Analysis of Fungichromin from Bacillus Strains

Chen Zheng, Liu Bo, Zhu Yujing, Pan Zhizhen, Liu Guohong(Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003)

Received:2015-08-18 Revised:2016-04-18 Accepted:2015-12-23 Online:2016-04-27 Published:2016-04-27

摘要/Abstract

摘要： 采用高效液相四级杆飞行时间质谱分析巴达维亚芽胞杆菌Bacillus bataviensis FJAT-10043发酵液中胞外代谢物的成分。运用安捷伦公司的MassHunter软件，对数据进行分子特征提取，并通过Metlin代谢物质谱数据库检索，获得总体代谢物的信息。在巴达维亚芽胞杆菌Bacillus bataviensis FJAT-10043发酵液中，用液相质谱检测到656种代谢物，通过Metlin谱库搜索得到初步鉴定结果的有121种。其中，喷他霉素是相对含量最高的成分。喷他霉素占该菌发酵液总代谢物相对含量的4.22%，匹配得分达到96.39，保留时间为2.7339 min。喷他霉素发现为巴达维亚芽胞杆菌来源的抗真菌抗生素的开发与利用提供理论依据。

关键词: 紫茎泽兰, 紫茎泽兰, 不同生境, 幼苗, 动态特征

Abstract: Extracellular metabolites of Bacillus bataviensis FJAT-10043 were analyzed by liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry. Molecular Feature Extraction (MFE) and database retrieval were applied to accessing the information of overall metabolites of Bacillus bataviensis FJAT-10043. As a result, 656 compounds were detected, and 121 of them were identified. Fungichromin from Bacillus bataviensis FJAT-10043 was analyzed and tested with the highest relative content. The match score of Fungichromin was 96.39, and the relative content was 4.22%. The retention time of this compound was about 2.7339 min. This result provided a theory basis for the development and utilization of the immunomodulator from Bacillus bataviensis.

陈峥,刘波,朱育菁,潘志针,刘国红. 芽胞杆菌中的喷他霉素检测与分析[J]. 中国农学通报, 2016, 32(12): 98-102.

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芽胞杆菌中的喷他霉素检测与分析

Detection and Analysis of Fungichromin from Bacillus Strains

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