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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (26): 60-64.doi: 10.11924/j.issn.1000-6850.casb16030175

所属专题： 生物技术

• 植物保护 农药 • 上一篇    下一篇

3种杀虫剂对榆紫叶甲成虫离体保护酶与解毒酶活性的影响

王 莹1,孙丽丽1,萨喜雅尔图2,王志英1   

  1. (1东北林业大学林学院,哈尔滨 150040；2内蒙古呼伦贝尔市林木种苗站,呼伦贝尔 021008）
  • 收稿日期:2016-03-23 修回日期:2016-05-19 接受日期:2016-05-25 出版日期:2016-09-21 发布日期:2016-09-21
  • 通讯作者: 王莹
  • 基金资助:
    黑龙江省科技计划项目(GA09B203-3)。

Three Kinds of Pesticides Affecting the Activities of Protective and Detoxifying Enzymesin Ambrostoma quadriimpressum Motsch in vitro

Wing Ying1, Sun Lili1, Saxiyaertu2, Wang Zhiying1   

  1. (1Colloge of Forestry, Northeast Forestry University, Harbin 150040; 2Forest and Seed Planting Station of Hulunbuir, Hulunbuir 021008)
  • Received:2016-03-23 Revised:2016-05-19 Accepted:2016-05-25 Online:2016-09-21 Published:2016-09-21

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摘要： 通过研究榆紫叶甲(Ambrostoma quadriimpressum Motsch)成虫，比较吡虫啉、灭幼脲、阿维菌素对其离体保护酶（SOD和CAT）和解毒酶（CarE和GST）活性的影响，为杀虫剂的合理使用提供基础资料和理论指导。取榆紫叶甲成虫制取酶液，将吡虫啉、灭幼脲、阿维菌素3种农药配置成5个浓度梯度0.001、0.010、0.100、1.000、10.000 mg/L，测其对离体保护酶和解毒酶活性的影响。试验结果表明：0.010 mg/L吡虫啉、0.001 mg/L灭幼脲对CAT活性的抑制作用较强，抑制率分别为49.62%、52.11%，阿维菌素对CAT活性的抑制作用较弱；阿维菌素对榆紫叶甲成虫离体SOD活性虽有一定的抑制作用，但抑制作用较弱，高质量浓度的吡虫啉(>0.010 mg/L)对SOD活性具有促进作用；阿维菌素对榆紫叶甲成虫离体CarE的活性具有抑制作用，而吡虫啉、灭幼脲对离体CarE的活性具有促进作用；3种农药对榆紫叶甲离体GST活性均有抑制作用，其中吡虫啉抑制作用最强。由此可知，离体保护酶和解毒酶的活性与杀虫剂的种类直接相关，不同类型的杀虫剂对其影响差异显著。

关键词: 食用菌, 食用菌, 栽培种, 细菌隐性污染, 基内菌丝分离, 菌种分离

Abstract: To provide basic data and theoretical guidance for the scientific use of pesticides, the effects of three kinds of pesticides on the activities of protective enzymes (SOD,CAT) and detoxifying enzymes (CarE, GST) in Ambrostoma quadriimpressum adult in vitro were studied. The enzyme solutions were prepared using Ambrostoma quadriimpressum Motsch adult. Different concentrations of imidacloprid, chlorbenzuron and abamectin were prepared, which were 0.001, 0.010, 0.100, 1.000, 10.000 mg/L. Then effects of the three kinds of pesticides on the enzymes were analyzed. The results showed that the activity of CAT was greatly inhibited by 0.010 mg/L imidacloprid solution and 0.001 mg/L chlorbenzuron, and the inhibitory rate was 49.62% and 52.11%, respectively. However, the activity of CAT was slightly inhibited by abamectin. The activity of SOD was slightly inhibited by abamectin. The activity of SOD was induced by high concentrations of imidacloprid (>0.010 mg/L). The activity of CarE was inhibited by abamectin while it was induced by imidacloprid and chlorbenzuron. The three kinds of pesticides all inhibited the activity of GST, but the imidacloprid showed the strongest inhibitory effect on GST. The results indicated that the activities of protective enzymes and the detoxifying enzymes in vitro were directly related to different kinds of pesticides, and the effects of the different type pesticides on the activities of in vitro enzymes were significantly different.