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中国农学通报 ›› 2016, Vol. 32 ›› Issue (34): 113-118.doi: 10.11924/j.issn.1000-6850.casb16060025

所属专题: 生物技术 园艺

• 植物保护 农药 • 上一篇    下一篇

草莓新品种‘晶玉’抗炭疽病相关的生理因子分析

曾祥国1,张 鹏1,2,韩永超1,向发云1,陈丰滢1,张庆华1,顾玉成1   

  1. (1湖北省农业科学院经济作物研究所,武汉 430064;2中南民族大学生命科学学院,武汉 430074)
  • 收稿日期:2016-06-06 修回日期:2016-11-22 接受日期:2016-09-01 出版日期:2016-12-15 发布日期:2016-12-15
  • 通讯作者: 顾玉成
  • 基金资助:
    湖北省农业科技创新中心资助项目“草莓新品种选育及设施栽培技术创新”(2016-620-000-001-014);湖北省公益性科技研究项目“湖北主要果树新品种选育与种质创新”(2013BBB09);湖北省科技基础条件平台建设项目“猕猴桃等浆果类果树种质资源共享服务平台”(2015BCE093)。

Physiological Factors Involved in Resistance of Strawberry Cultivar ‘Jingyu’ to Anthracnose

Zeng Xiangguo1, Zhang Peng1,2, Han Yongchao1, Xiang Fayun1,   

  1. Chen Fengying1, Zhang Qinghua1, GuYucheng1(1Institute of Industrial Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064;2College of Life Science, South-Central University for Nationalities, Wuhan 430074)
  • Received:2016-06-06 Revised:2016-11-22 Accepted:2016-09-01 Online:2016-12-15 Published:2016-12-15

摘要: 为研究草莓新品种‘晶玉’抗炭疽病的生理机制,以抗炭疽病草莓新品种‘晶玉’为研究材料,高抗品种‘甜查理’以及易感品种‘丰香’和‘红颜’为对照,通过检测接种炭疽菌后不同时间点的木质素、总酚、绿原酸、阿魏酸含量以及苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)活力的变化差异,发现感染炭疽病后,上述生理因子在抗、感品种中均有不同程度的增加,并且抗病品种中上述生理因子的峰值均高于感病品种,说明接菌处理后这些生理因子含量的增加可能是导致草莓产生抗炭疽病特性的机制之一。研究初步揭示,高木质素、总酚、绿原酸、阿魏酸含量以及PAL、PPO活力是与‘晶玉’具有较强抗炭疽病能力相关的生理因子,为深入研究‘晶玉’抗炭疽病的生物学机制提供了参考。

关键词: CVM, CVM, 吉林省湿地, 生物多样性维持, 支付意愿(WTP), 价值评估

Abstract: In order to study the physiological mechanisms of the resistance of a new strawberry cultivar ‘Jingyu’ to anthracnose, we used ‘Jingyu’ as research object and high-anthracnose-resistant strawberry ‘Sweet Charlie’, anthracnose-susceptible strawberry ‘Benihoppe’ and ‘Toyonoka’ as controls, to detect the changes of the content of lignin, total phenols, chlorogenic acid and ferulic acid, along with the changes of the activity of phenylalanin ammonialyase (PAL) and polyphenol oxidase (PPO) at the different times in the leaves after inoculated with Colletotrichum gloeosporioides. The results showed that the content or activity of the above physiological factors were improved after inoculated with C. gloeosporioides, and the highest content or activity of these factors in anthracnose-resistant strawberry cultivars were higher than that in anthracnose-susceptible cultivars. These results suggested that the resistance of strawberry ‘Jingyu’ to anthracnose was involved in the high content of lignin, total phenols, chlorogenic acid and ferulic acid, along with the high activity of PAL and PPO. The study laid a foundation for further research on the resistance mechanism of the new strawberry cultivar ‘Jingyu’ to anthracnose.