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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (9): 31-36.doi: 10.11924/j.issn.1000-6850.casb16100074

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

转CpFATB 基因苜蓿后代抗寒性分析

冯鹏,孙力,申晓慧,李如来,郑海燕,李志民,李增杰,姜成,郭伟   

  1. 黑龙江省农业科学院佳木斯分院;黑龙江省农业科学院博士后工作站;佳木斯大学生命学院 黑龙江 佳木斯,黑龙江省农业科学院佳木斯分院,黑龙江省农业科学院佳木斯分院,黑龙江省农业科学院佳木斯分院,黑龙江省农业科学院佳木斯分院,黑龙江省农业科学院佳木斯分院,黑龙江省农业科学院佳木斯分院,佳木斯大学生命学院 黑龙江 佳木斯,黑龙江省农业科学院佳木斯分院
  • 收稿日期:2016-10-18 修回日期:2016-12-13 接受日期:2016-12-23 出版日期:2017-03-28 发布日期:2017-03-28
  • 通讯作者: 冯鹏
  • 基金资助:
    中国博士后科学基金“CPFAT基因在紫花苜蓿中的表达特性及提高抗寒性研究”(2013M541423)。

Cold Resistance of Transgenic Alfalfa with CpFATB Gene

  • Received:2016-10-18 Revised:2016-12-13 Accepted:2016-12-23 Online:2017-03-28 Published:2017-03-28

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摘要： 为明确CpFATB基因在苜蓿中的表达特点及生理功能,获得苜蓿抗寒育种的基因资源。本实验通过根癌农杆菌介导将腊梅硫酯酶基因(CpFATB)转入龙牧806、WL319HQ和肇东3个苜蓿品种中,对其后代进行抗寒性鉴定。结果表明,三个苜蓿品种转CpFATB基因SP3代(第三代基因修饰材料)种子发芽率、简化活力指数均高于各自对照,其中T龙牧806最高,达到87.79%、208.19；转基因植株叶绿素a、叶绿素b及叶绿素总量显著低于其各自对照苜蓿品种,根系可溶性糖、可溶性蛋白、游离脯氨酸含量显著高于其各自对照苜蓿品种；转CpFATB基因植株高度和分枝数与其各自对照差异不显著,而越冬率显著高于其各自对照品种,T龙牧806越冬率最高,达到96.60%。转CpFATB基因促进龙牧806、WL319HQ、肇东苜蓿SP3代种子萌发；抑制苜蓿叶片叶绿素合成。越冬前促进根系可溶性糖、可溶性蛋白、游离脯氨酸含量积累,POD、SOD活性增强,提高了翌年越冬率,说明外源CpFATB基因有助于苜蓿抗寒性的提高。

关键词: 雾霾, 雾霾, 变化特征, 等级分布

Abstract: The aims are to clarify the expression characteristics and physiological function of CpFATB (Chimonanthus praecox chloroplast acyl-ACP thioesterase) in alfalfa, and obtain the cold resistance resources of alfalfa for breeding. CpFATB gene was transferred to‘Longmu 806’,‘WL319HQ’,‘Zhaodong’by agrobacterium tumefaciens, and the ability of cold resistance of the third GMOs’generation was tested. The results showed that the germination rate and simple vigor index of SP3 generation were significantly higher than that of the control, and that of T‘Longmu 806’was the highest which achieved 87.79% and 208.19. Chlorophyll a, chlorophyll b and total chlorophyll of transgenic CpFATB plant were lower than that of the control; the soluble sugar, soluble protein and free proline were significantly higher than that of the control; the plant height and branch number had no significant difference between transgenic CpFATB plant and the control; the overwinter rate was significantly greater than that of the control, and the overwinter rate of T ‘Longma 806’was the highest which reached 96.60%. CpFATB gene promoted seed germination of‘Longma 806’,‘WL319HQ’and‘Zhaodong’SP3 generation, while inhibited the chlorophyll biosynthesis. It promoted accumulation of soluble sugar, soluble protein, free proline before overwinter, enhanced the activities of POD and SOD, and then improved the overwinter rate of alfalfa, which indicated that CpFATB gene improved the ability of cold resistance of alfalfa.