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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (1): 142-147.doi: 10.11924/j.issn.1000-6850.casb16110096

• 植物保护 农药 • 上一篇    下一篇

甲氰菊酯降解菌的培养基优化

张正一,杨丽莉,梁爽,王鑫宏,张浩   

  1. 吉林农业大学资源与环境学院,吉林省农业技术推广总站,吉林农业大学,吉林农业大学,吉林农业大学
  • 收稿日期:2016-11-18 修回日期:2017-12-13 接受日期:2017-01-20 出版日期:2018-01-12 发布日期:2018-01-12
  • 通讯作者: 张浩
  • 基金资助:
    国家重点研发计划支持项目(2016YFD0200203)。

Optimization of Culture Medium for Fenpropathrin-degrading Bacteria

张正一,杨丽莉,梁爽,王鑫宏 and 张浩   

  • Received:2016-11-18 Revised:2017-12-13 Accepted:2017-01-20 Online:2018-01-12 Published:2018-01-12

摘要： 本研究旨在优化甲氰菊酯降解菌在培养基中的生长条件，探索基础盐培养基配方为该菌的大量繁殖提供理论依据。从淤泥中富集分离筛选出一株甲氰菊酯降解菌(Y1)并保存，经过鉴定该菌属于蜡样芽孢杆菌(Bacillus cereus)。通过单因素试验测定OD600值确定了该菌繁殖的最适碳源和氮源，正交试验明确最佳基础盐培养基。结果表明：最适碳源与氮源分别为蔗糖和蛋白胨，最适浓度分别为0.15%和2.5%；最佳基础盐培养基配方为：pH 6.0，蔗糖0.15%，蛋白胨2.5%，K2HPO4 0.001%，KH2PO4 0.01%，MgSO4· 7H2O 0.1%，NaCl 0.01%。在优化后的基础盐培养基中Y1 能够大量繁殖，为该菌在甲氰菊酯污染的土壤中进行修复奠定了基础。

关键词: 四川, 四川, 烟叶, SWOT模型, 竞争力

Abstract: The aims are to improve the growth condition of fenpropathrin-degrading bacteria in the growth medium, explore the basal salt medium formula and provide the basis for mass propagation of fenpropathrindegrading bacteria. In this paper, a strain of degrading bacteria (Y1) was preconcentrated and screened from the sludge and then preserved, which was identified as Bacillus cereus. OD600 was measured through the singlefactor test, and the optimal carbon and nitrogen sources were determined. The optimal basal salt culture medium was obtained through the orthogonal test. The results showed that optimal carbon and nitrogen sources were sucrose and peptone, and the optimal concentrations were 0.15% and 2.5%. The best basal salt medium was pH 6.0, sucrose 0.15%, peptone 2.5%, K2HPO4 0.001%, KH2PO4 0.01%, MgSO4·7H2O 0.1%，NaCl 0.01%. Y1 could multiply in the optimized basal salt medium, which established the foundation of its remediation for soil contaminated by fenpropathrin.

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