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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (2): 74-78.doi: 10.11924/j.issn.1000-6850.casb16120020

• 植物保护 农药 • 上一篇    下一篇

不同杀菌剂对三七黑斑病菌的室内毒力测定

包媛媛,赵娟秀,杨加艳,王官民,毛忠顺,杨莉,冯光泉   

  1. 云南农业大学食品科学技术学院,云南农业大学食品科学技术学院,云南农业大学食品科学技术学院,云南农业大学食品科学技术学院,文山学院文山三七研究院;云南省三七工程技术研究中心,文山学院文山三七研究院;云南省三七工程技术研究中心,文山学院文山三七研究院;云南省三七工程技术研究中心
  • 收稿日期:2016-12-04 修回日期:2017-04-06 接受日期:2017-04-19 出版日期:2018-01-23 发布日期:2018-01-23
  • 通讯作者: 冯光泉
  • 基金资助:
    云南省科技创新强省计划项目“三七种植及初加工的农残、重金属安全控制技术与集成示范”(2014AE014)；云南省高校科技创新团队建设 计划项目“云南省高校三七安全无污染种植加工关键技术科技创新团队”(IRTSTYN 2014-22)；文山学院创新团队建设项目“植物病理学三七病理学 创新团队”(A1000036)；云南省教育厅重点基金“氟硅唑在三七上残留消解动态及皂苷合成相关性的研究”(2015Z096)；国家大学生创新创业计划“氟 硅唑在三七上残留动态及与皂苷含量相关性的研究”(20150676003)；云南省教育厅研究生基金“氟硅唑在三七上残留消解动态及皂苷合成相关性的 研究”(2015J062)。

Toxicity Determination of Different Fungicides on Alternaria panax

  • Received:2016-12-04 Revised:2017-04-06 Accepted:2017-04-19 Online:2018-01-23 Published:2018-01-23

摘要： 三七黑斑病是三七的主要叶部病害,年均发病率在20%-35%之间,严重时可达90%以上。为筛选可用于田间防治化学杀菌剂,选取福美双、氟硅唑、苯醚甲环唑、丙环唑、戊唑醇、百菌清、烯唑醇、异菌脲、代森锰锌、多菌灵和苯酰菌胺共11种化学杀菌剂原药,采用菌丝生长抑制法和孢子萌发抑制法测定了其对三七黑斑病菌的抑制作用。结果表明,苯醚甲环唑对病原菌菌丝的EC50为0.004mg.L-1,是供试杀菌剂中最低的,表明其对三七黑斑病菌敏感；丙环唑、戊唑醇、氟硅唑和烯唑醇的抑制作用较好；多菌灵的抑制作用较差,EC50值在800 mg.L-1以上。孢子萌发抑制作用实验结果表明,代森锰锌对病原菌的EC50值最小,为0.0067mg.L-1,抑菌活性最高；百菌清和福美双的EC50值分别为1.2855 mg.L-1、5.2379mg.L-1,抑制活性次之；丙环唑、氟硅唑、苯醚甲环唑和异菌脲的EC50值10.6469mg.L-1～25.0169mg.L-1之间,抑菌活性一般；苯酰菌胺和多菌灵的EC50值分别为106.1958mg.L-1和199.9349 mg.L-1,抑菌活性差；戊唑醇和烯唑醇的EC50值为在300 mg.L-1,抑菌活性较差。

关键词: 江西省, 江西省, DEA-Malmquist指数法, 全要素生产率

Abstract: Alternaria panax Wheltz is the main leaf disease of Panax notoginseng (Burk.) F H Chen. The average incidence of Alternaria panax ranges from 20% to 35%, even up to 90% in some plantations. To screen the appropriate fungicide for field control, eleven fungicides, including thiram, flusilazle, difenoconazole, propiconazole, debuconazole, chlorothalonil, diniconazole, iprodione, mancozeb, carbendazim, and zoxamide, were selected to determine their toxicity to Alternaria panax with mycelium growth inhibitory method and spore germination inhibitory method. The results showed that EC50 of difenoconazole for pathogen hyphae was 0.004 mg/L, which was the minimum in all fungicides, indicating that difenoconazole was sensitive to Alternaria panax. Propiconazole, debuconazole, flusilazole and diniconazole had better inhibitory activity. Iprodione, chlorothalonil, thiram, zoxamide and mancozeb had worse inhibition activity, While carbendazim had the worst inhibitory activity with EC50 value over 800 mg/L. Spore germination inhibition results showed that mancozeb had the best inhibition activity with the minimum EC50 value of 0.0067 mg/L, while chlorathalonil and thiram had the second inhibition activity with EC50 value as 1.2855 mg/L and 5.2379 mg/L, respectively. Propiconazole, flusilazole, difenoconazole, and iprodione had normal inhibition activity with EC50 value ranged from 10.6469 mg/L to 25.0169 mg/L. Zoxamide and carbendazim had less inhibition activity with EC50 valueas 106.1958 mg/Land 199.9349 mg/L, respectively. EC50 value of debuconazole and diniconazole to Alternaria panax were 300 mg/L, and the inhibition activity was the worst.