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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (26): 136-140.doi: 10.11924/j.issn.1000-6850.casb17010078

所属专题： 生物技术； 水产渔业

• 水产 渔业 • 上一篇    下一篇

葛根素对草鱼非特异性免疫机能和肝胰脏组织结构的影响

饶毅   

  1. 江西省水产科学研究所
  • 收稿日期:2017-01-16 修回日期:2017-05-03 接受日期:2017-06-08 出版日期:2017-09-19 发布日期:2017-09-19
  • 通讯作者: 饶毅
  • 基金资助:
    江西省现代农业科研协同创新专项“草鱼池塘健康生态高效养殖技术研究”(JXXTCX2015004-006)；江西省现代农业产业技术体系专项 (JXARS-03)；江西省农牧渔科研指导性计划项目“不同饲料投喂量下生物絮团技术对草鱼生长的影响”(2016123-17)。

Puerarin Affecting Non-specific Immune Function and Hepatopancreas Structure of Grass Carp (Ctenopharyngodon idellus)

  • Received:2017-01-16 Revised:2017-05-03 Accepted:2017-06-08 Online:2017-09-19 Published:2017-09-19

摘要： 为研究葛根提取物葛根素对草鱼非特异性免疫指标和肝胰脏组织结构的影响，在基础饲料（对照组）中分别添加10mg/kg、50 mg/kg、250 mg/kg、1250 mg/kg的葛根素，饲养平均体重4.52±0.22g的草鱼。经28d的饲养，结果表明，与对照组比较，10 mg/kg葛根素添加组碱性磷酸酶活性显著高于对照组（P<0.05），10 mg/kg和50mg/kg葛根素添加组超氧化物歧化酶显著高于对照组（P<0.05），溶菌酶各组间无显著差异（P>0.05）。肝胰脏指数各试验组与对照组无显著差异（P>0.05）。对照组和各试验组之间肝胰脏组织结构差异也不显著。饲喂结束后，利用嗜水气单胞菌人工感染草鱼，各试验组的相对免疫保护率都提高，但10 mg/kg葛根素添加组对草鱼的保护率显著高于对照组（P<0.05），免疫保护率达73.33%。试验表明，在饲料中添加一定量的葛根素（10 mg/kg）能提高草鱼的非特异性免疫机能，且不会造成肝胰脏组织结构的损伤。

关键词: 鸢尾, 鸢尾, 鸢尾酮, 萃取工艺, 响应面法, 优化

Abstract: The study aims to research the effects of adding puerarin in diets on non-specific immune function and hepatopancreas structure of grass carp (Ctenopharyngodon idellus). C. idellus with body weight of (4.52±0.22) g were fed with basal diet without (control group) or with 10, 50, 250, and 1250 mg/kg puerarin extract for 28 days. The results showed that the serum AKP activity significantly increased in the group with 10 mg/kg puerarin addition compared with that in control group (P<0.05), and SOD activity significantly increased in the groups with 10 and 50 mg/kg puerarin addition (P<0.05). However, LZM activity was not affected in all the tested groups. Hepatopancreas index and structure was not significantly different between tested groups and control group. After the feeding experiment, C. idellus were infected with Aeromonas hydrophila. Survival rates in the test groups all increased, and the increase was significant for the group with 10 mg/kg puerarin addition (immune protective rate of 73.33%, P<0.05). The results indicate that non-specific immune function can be improved by adding 10 mg/kg puerarin in diet, which cannot lead to hepatopancreas injury.