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中国农学通报 ›› 2022, Vol. 38 ›› Issue (3): 102-109.doi: 10.11924/j.issn.1000-6850.casb2021-0229

• 生物科学 • 上一篇    下一篇

基于响应面法优化印度梨形孢培养基

李佳欢1(), 王晓慧1, 姜明国2, 宋福强1, 常伟1()   

  1. 1黑龙江大学生命学院/黑龙江省寒地生态修复与资源利用重点实验室,哈尔滨 150080
    2广西民族大学海洋与生物技术学院/广西多糖材料与改性重点实验室,南宁 530008
  • 收稿日期:2021-03-09 修回日期:2021-06-13 出版日期:2022-01-25 发布日期:2022-02-25
  • 通讯作者: 常伟
  • 作者简介:李佳欢,女,1997年出生,黑龙江齐齐哈尔人,本科,主要从事微生物生态学研究。通信地址:150080 黑龙江省哈尔滨市南岗区学府路74号 黑龙江大学生命科学学院,Tel:0451-86609453,E-mail: lijiahuan_1998@163.com
  • 基金资助:
    黑龙江省自然科学基金研究团队项目“寒区农业土壤面源污染微生物调控技术的研究”(TD2019C002);广西科技基地和人才专项“海洋生物资源保护与利用国际联合实验室建设”(桂科AD18281066)

Optimization of Culture Medium of Serendipita indica Based on Response Surface Methodology

LI Jiahuan1(), WANG Xiaohui1, JIANG Mingguo2, SONG Fuqiang1, CHANG Wei1()   

  1. 1Key Laboratory of Ecological Restoration and Resource Utilization in the Cold Region of Heilongjiang Province, Heilongjiang University, Harbin 150080
    2Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530008
  • Received:2021-03-09 Revised:2021-06-13 Online:2022-01-25 Published:2022-02-25
  • Contact: CHANG Wei

摘要:

印度梨形孢(Serendipita indica)是一种能在人工培养基上培养的根内生真菌。为了提高S. indica的生长速率,进一步优化S. indica培养基。本研究通过单因素实验探究6种影响因子对S. indica生长的影响,并通过响应面法对S. indica培养基进行精细优化。结果表明,S. indica菌株生长的最适温度为31±2℃,最适pH为6.9±0.2,最佳碳源为蔗糖,最佳氮源为NaNO3,最佳无机盐为MgSO4·7H2O。应用RSM最终确定S. indica菌株优化后的培养基配方如下:MgSO4·7H2O为0.824 g/L、KH2PO4为1.118 g/L、NaNO3为1.055 g/L、NaCl为0.03 g/L、CaCl2为0.05 g/L、蔗糖为10 g/L;在此条件下,S. indica的平均生长速率为1.8083 cm/d,远大于原MMN培养基继代培养的平均生长速率。该研究通过RSM优化S. indica培养基成分,提高S. indica生长速率,为S. indica菌株规模化生产并在田间试验中的应用奠定基础。

关键词: 印度梨形孢, 培养基, 响应面法, 单因素优化, 生长速率

Abstract:

The root endophytic fungus Serendipita indica can be cultured on artificial medium. The present study aims at optimizing the medium to enhance the growth rate of S. indica. In this experiment, the single-factor experiment was selected for evaluating the effect of six parameters on the growth rate of S. indica, and the optimization design of the medium was performed by the response surface methodology (RSM). The results showed that the optimum temperature was 31±2℃, the optimum pH was 6.9±0.2, the best carbon source was sucrose, the best nitrogen source was NaNO3, and the best inorganic salt was MgSO4·7H2O. The optimal medium formulation was determined by RSM as following: MgSO4·7H2O 0.824 g/L, KH2PO4 1.118 g/L, NaNO3 1.055 g/L, NaCl 0.03 g/L, CaCl2 0.05 g/L and sucrose 10 g/L. In the optimal medium, the average growth rate of S. indica reached 1.8083 cm/d, which was greatly faster than that in the original MMN medium. In this study, medium optimization based on RSM improved the growth rate of S. indica, which laid a foundation for the large-scale production of S. indica and its application in field trials.

Key words: Serendipita indica, medium, response surface method, single factor optimization, growth rate

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