Brevibacillus laterosporus BL-21和Bacillus subtilis HNDF2共培养与纯培养抗菌代谢产物的对比分析

doi:10.11924/j.issn.1000-6850.casb17010103

中国农学通报 ›› 2017, Vol. 33 ›› Issue (22): 118-125.doi: 10.11924/j.issn.1000-6850.casb17010103

Brevibacillus laterosporus BL-21和Bacillus subtilis HNDF2共培养与纯培养抗菌代谢产物的对比分析

周立梅,张淼,徐德阳,杨智越,杜春梅

黑龙江大学生命科学学院501室,黑龙江大学生命科学学院501室,黑龙江大学生命科学学院501室,黑龙江大学生命科学学院501室,黑龙江大学生命科学学院501室

收稿日期:2017-01-19 修回日期:2017-07-19 接受日期:2017-03-25 出版日期:2017-08-21 发布日期:2017-08-21
通讯作者: 杜春梅
基金资助:
国家自然科学基金项目（面上项目，重点项目，重大项目）

Comparative Analysis of Co-culture and Pure Culture of Antibacterial Metabolites of Brevibacillus laterosporus BL-21 and Bacillus subtilis HNDF2

Received:2017-01-19 Revised:2017-07-19 Accepted:2017-03-25 Online:2017-08-21 Published:2017-08-21

摘要/Abstract

摘要： 旨在对比分析Brevibacillus laterosporus BL-21 和Bacillus subtilis HNDF2 纯培养液和共培养液的差异，为研究两者的生态学关系和发现新抗菌物质提供参考。采用杯碟法检测抗菌活性，采用薄层层析、SDS-PAGE 电泳以及HPLC 分析代谢产物的差异。结果表明，共培养液对尖孢镰刀菌(Fusarium oxysporum)抗菌活性最高时的抑菌圈直径达到(28.50±0.5) mm，与B. laterosporus BL-21 和B. subtilis HNDF2 纯培养液的最高抑菌活性相比，分别提高了18.75%和83.90%。代谢产物检测结果表明，共培养液与B. laterosporus BL-21 纯培养液的相似度较高，与B. subtilis HNDF2 纯培养液的差别较大，并且含有各纯培养液中没有的新物质。2 株菌的纯培养液和共培养液在抗菌活性和组分上存在显著差异，有必要对共培养液中的抗菌物质进行深入分析。

关键词: 泰山椴, 泰山椴, 扦插, 生根

Abstract: The aim of the study is to compare and analyze the differences between pure culture and co-culture metabolites ofBrevibacillus laterosporus BL-21 andBacillus subtilis HNDF2, and lay a foundation for further research on ecological relationship between the two strains and find new antibacterial substances from coculture broth. The antibacterial activity was detected by cylinder plate method. The differences of metabolites were analyzed by thin layer chromatography, SDS-PAGE and HPLC. The results showed that the maximum bacteriostatic diameter of co- culture broth against Fusarium oxysporum could reach (28.50 ± 0.5) mm, compared with that of pure culture broth ofB. laterosporus BL-21 andB. subtilis HNDF2, the antagonistic activity increased by 18.75% and 83.90%, respectively. The results of metabolites determination showed that the co-culture broth had a high similarity to the pure culture broth of B. laterosporus BL-21, but it had significant differences to the pure culture broth ofB. subtilis HNDF2, and some new substances were detected in co- culture broth which did not exist in each pure culture broth. There were significant differences in antifungal activity and components of metabolites between pure culture broth and co-culture broth of the two strains, and it was necessary to further analyze the antimicrobial substances in co-culture broth.

周立梅,张淼,徐德阳,杨智越,杜春梅. Brevibacillus laterosporus BL-21和Bacillus subtilis HNDF2共培养与纯培养抗菌代谢产物的对比分析[J]. 中国农学通报, 2017, 33(22): 118-125.

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Brevibacillus laterosporus BL-21和Bacillus subtilis HNDF2共培养与纯培养抗菌代谢产物的对比分析

Comparative Analysis of Co-culture and Pure Culture of Antibacterial Metabolites of Brevibacillus laterosporus BL-21 and Bacillus subtilis HNDF2

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