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中国农学通报 ›› 2018, Vol. 34 ›› Issue (21): 101-107.doi: 10.11924/j.issn.1000-6850.casb17050092

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

纳米碳腐植酸保水肥对柑橘田土壤细菌群落结构及柑橘生长的影响

柯 超,张世伟,马 筠,钟 建,黄占斌   

  1. 中国矿业大学北京化学与环境工程学院,北京新源国能科技集团股份有限公司,中国矿业大学北京化学与环境工程学院,中国科学院微生物研究所,中国矿业大学北京化学与环境工程学院,中国矿业大学北京化学与环境工程学院
  • 收稿日期:2017-05-19 修回日期:2018-06-26 接受日期:2017-07-24 出版日期:2018-07-26 发布日期:2018-07-26
  • 通讯作者: 黄占斌
  • 基金资助:
    三峡后续工作科研项目“三峡库区农业面源污染控制纳米碳添加肥与生物组合技术应用研究”(2015HXKY2-4-2);国家自然科学“高分子保水剂对土壤水肥保持和重金属固化多重效应的机理研究”(41571303)

Effects of Nano-carbon Water-retain Fertilizer on Soil Bacterial Community Structure and Citrus Growth in Citrus Field

柯 超,张世伟,, and 黄占斌   

  • Received:2017-05-19 Revised:2018-06-26 Accepted:2017-07-24 Online:2018-07-26 Published:2018-07-26

摘要: [目的]为揭示新型纳米炭腐植酸保水肥(CSF)减量化对土壤微生物种群结构及柑橘生长的影响。[方法]本研究以三峡库区万州柑橘园为对象,以常规施肥为对照(KC1)),分别在CSF减量0%(KC2)、10%(KC3)、20%(KC4)、30%(KC5)和40%(KC6)处理下对土壤细菌群落结构及柑橘产量、品质进行分析。[结果]结果显示,KC6的 Observed-species、Shannon指数、Chao1指数、PD_whole_tree总体高于KC1,并与KC2基本持平;在科水平上,KC5黄色单胞菌科(Xanthomonadaceae)丰度最高;与KC1相比,KC3,KC4,KC6中的黄色单胞菌科(Xanthomonadaceae)均明显降低,KC6处理后亚硝化单胞菌科(Nitrosomonadaceae)、假单胞菌科(Pseudomonadaceae)较KC1有所提高;在属水平上,不同减量处理的鞘脂单胞菌属(Sphingomonas)基本低于KC1,但伯克氏菌属(Burkholderia)、假单胞菌属(Pseudomonas)基本高于KC1,细菌群落相似性聚类分析发现,各处理间相似性较小。CSF减量施肥后柑橘产量均有所增加,CSF减量施肥30%,增加了约4.50%,CSF减量施肥40%后减少了4.14%。施用CSF复合肥后,柑橘品质在CSF常量施肥与减量10%,减量40%时并无显著变化,而在减量20%、30%时显著下降。[结论]以上结果表明,CSF复合肥减量化施肥处理改变了土壤细菌群落结构的多样性及柑橘产量、品质。

关键词: 蔬菜, 蔬菜, 水稻, 轮作, 氮磷, 积累

Abstract: [Objective]In order to reveal the effect of reducing the amount of novel nano-carbon humic acid water-retaining fertilizer (CSF) on soil microbial communities structure and citrus growth.[Method] In this study, conventional fertilization was as the control (KC1) in Wanzhou citrus orchard of Three Gorges Reservoir area. Reduction of CSF 0%(KC2), 10% (KC3), 20% (KC4), 30% (KC5) and 40% (KC6) were used to analyze the changes of soil bacterial community structure, citrus yield and quality. [Result]The results showed that the Observed-species, Shannon index, Chao1 index and PD_whole_tree of KC6 were higher than those of KC1, and were the same as KC2. The abundance of Kanthocyanidaeae was the highest in KC5. Compared with KC1, the Kanthoxicaceaeaceae in KC3, KC4 and KC6 were significantly decreased, and the levels of Nitrosomonadaceae and Pseudomonasaceae were higher than that of KC1 after the treatment of KC6. Sphingomonas was lower than that of KC1, but Burkholderia and Pseudomonas were significantly higher than those of KC1.. It was found that the similarity among treatments was small after bacterial community similarity clustering analysis, and the yield of citrus was reduced by CSF after fertilization, when the fertilization was reduced by 30%, the yield was increased by 4.50%, whist the CSF was reduced of 40% the yield was reduced of 4.14%. After the application of CSF compound fertilizer, citrus quality in the CSF constant fertilization and reduction of 10% and 40%, the quality did not significantly change, and in the reduction of 20%, 30%, the quality was significantly decreased.[Conclusion]The results showed the diversity of soil bacterial community structure, the yield and quality of citrus were changed with the reduction of CSF fertilization.

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