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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (17): 115-121.doi: 10.11924/j.issn.1000-6850.casb17060110

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

酶法水解牦牛血制备蛋白多肽粉的技术研究

韩学燕,曹效海   

  1. 青海大学,青海大学
  • 收稿日期:2017-06-25 修回日期:2017-08-27 接受日期:2017-09-10 出版日期:2018-06-20 发布日期:2018-06-20
  • 通讯作者: 曹效海
  • 基金资助:
    2014 年青海省科技厅科技支撑项目“青藏高原动物血液综合利用技术产业化”(2014-GX-136A)。

Hydrolysis of Yak Blood Protein into Polypeptide with Protease

  • Received:2017-06-25 Revised:2017-08-27 Accepted:2017-09-10 Online:2018-06-20 Published:2018-06-20

摘要： 摘 要：[目的][方法]利用中性蛋白酶将牦牛血红蛋白水解，探讨各因素对中性蛋白酶水解牦牛血红蛋白的影响以及水解度的关系，并对酶解液进行了活性炭脱色效果的研究。通过单因素和正交试验（L16(45)），[结果]确定了中性蛋白酶水解血红蛋白的适宜条件为 pH 7.0，温度 45℃，酶底物浓度比 4000 U/g 蛋白质液，底物浓度 5%，酶解时间 7 h。通过正交试验，确定酶解液的最佳脱色工艺条件为活性炭用量 4%，脱色温度 75℃，pH 5.0，脱色时间 60 min。

关键词: 低温, 低温, 保鲜, 功能性质

Abstract: The yak hemoglobin from blood was hydrolyzed in order to produce protein polypeptide with neutral protease. The factors influencing neutral protease to hydrolyze yak hemoglobin and their relationships with hydrolysis degree were explored by single factor experiments and orthogonal design [L16(45)]. And the effect of activated carbon on decolorization was analyzed. The results showed that the optimum hydrolysis conditions were as follows: pH 7.0, temperature 45℃, enzyme activity 4000 U/g substrate, substrate concentration 5%, hydrolysis duration 7 h. The optimum parameters for decolorization were determined to be active carbon consumption of 4%, temperature of 75℃, pH 5.0 and decolorization duration of 60 min.