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中国农学通报 ›› 2018, Vol. 34 ›› Issue (12): 125-131.doi: 10.11924/j.issn.1000-6850.casb17090055

所属专题: 园艺

• 食品 营养 检测 安全 • 上一篇    下一篇

红树莓花青素的微波辅助提取研究

刘莹,高涵,王丽霞,田益玲   

  1. 河北农业大学食品科技学院,河北农业大学食品科技学院,河北农业大学食品科技学院,河北农业大学食品科技学院
  • 收稿日期:2017-09-11 修回日期:2017-12-12 接受日期:2017-12-22 出版日期:2018-05-03 发布日期:2018-05-03
  • 通讯作者: 田益玲
  • 基金资助:
    河北省科技计划项目( No. 16226806D-1);河北省食品科学与工程学科“双一流”建设项目(No. 2016SPGCA18)

Anthocyanin extracted from red raspberry by MAE

  • Received:2017-09-11 Revised:2017-12-12 Accepted:2017-12-22 Online:2018-05-03 Published:2018-05-03

摘要: 目的:红树莓花青素的微波辅助提取及其体外清除自由基能力。方法:花青素提取工艺采用中心试验设计响应面试验设计,抗氧化能力测定通过了清除羟自由基、ABTS和DPPH进行评判。结果:红树莓花青素的最佳提取工艺为:微波强度600W,微波时间40S,液料比60︰1,酸化乙醇浓度的体积比为20:80,浸泡时间10min。在此条件下树莓花青素的预测含量为0.055mg/g,实际测得花青素的含量为0.054mg/g。

关键词: ‘大同34号’谷子, ‘大同34号’谷子, 干旱胁迫, 种子萌发, 耐旱性

Abstract: Our objective is to extract anthocyanin from Red Raspberry by microwave assisted extraction (MAE) and study the ability of scavenging free radicals in vitro. We design the experiment by central composite design. By measuring the ability of scavenging hydroxyl free radicals, ABTS and DPPH, we can determine their antioxidant capacity. The result is that after soaking 10min, the optimal conditions for MAE were using 20:80 acid ethanol as solvent with the material ratio of 1:60(w/v), and extracting for 40s under microwave power of 600W. In this condition, the predicted content of anthocyanin is 0.055mg/g, while the true content is 0.054mg/g.