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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (2): 79-83.doi: 10.11924/j.issn.1000-6850.casb17090086

• 植物保护 农药 • 上一篇    下一篇

毛细管电泳技术检测有机杂环类除草剂方法的研究

阳仲斌#,晏姣,戴清香,谭显胜,李甲枚,金晨钟,胡军和   

  1. 农业与生物技术学院,农业与生物技术学院,农业与生物技术学院,农业与生物技术学院,农业与生物技术学院,农业与生物技术学院,农业与生物技术学院
  • 收稿日期:2017-09-15 修回日期:2017-10-16 接受日期:2017-10-25 出版日期:2018-01-23 发布日期:2018-01-23
  • 通讯作者: 胡军和
  • 基金资助:
    湖南省教育厅创新平台开放基金项目“LC-MS方法检测五种有机杂环类除草剂残留的研究”(17K050)；湖南省研究生科研创新项目“喹啉 羧酸类除草剂毛细管电泳检测方法的研究”(CX2016B676)；湖南人文科技学院“英才支持计划”项目。

Detection Methods of Organic Heterocyclic Herbicides by Capillary Electrophoresis Technology

晏姣,,,, and   

  • Received:2017-09-15 Revised:2017-10-16 Accepted:2017-10-25 Online:2018-01-23 Published:2018-01-23

摘要： 毛细管电泳技术(CE),经过几十年的不断发展与改进,已经在农药残留领域被广泛应用,但是有关有机杂环类除草剂的毛细管电泳检测未见报道,其检测的方法技术体系有待研究。为此,通过筛选和优化CE技术的相关检测参数,建立了五种有机杂环类除草剂(西玛津、氰草津、西草净、扑草净、特丁津)的毛细管电泳检测方法(以10 mmol/L硼砂做为运行缓冲液,其PH为9.5；以30 mmol/L十二烷基硫酸钠,7.5%甲醇溶液为最佳分离介质；运行条件的设置为：进样压力为0.5 psi,进样时间为5s,分离电压为15 KV,毛细管柱温为：25 ℃,检测波长为221 nm),最终可以实现一次性对五种有机类杂环类除草剂进行高效、快速和准确的检测。

关键词: 沂河流域, 沂河流域, 土壤侵蚀, 分布特征

Abstract: Capillary electrophoresis (CE) has been widely used in the field of pesticide residues after several decades of continuous development and improvement. However, capillary electrophoresis detection of organic heterocyclic herbicides has not yet been studied. In this paper, CE determination methods of five organic heterocyclic herbicides (Simazine, Cyanazine, Simetryn, Prometryn and Terbutylazine) were established by screening CE detection parameters, 10 mmol/L borax was used as the running buffer, pH 9.5, 30 mmol/L sodium dodecyl sulfate and 7.5% methanol solution were used as the best separation medium; other conditions were injection pressure of 0.5 psi, injection time of 5 s, separation voltage of 15 kV, capillary column temperature of 25℃, detection wavelength of 221 nm. The efficient, fast and accurate detection of five kinds of organic heterocyclic herbicides could be achieved in one-time.