关键词: 木薯, 木薯, 淀粉磷酸化酶, 蛋白表达, 单克隆抗体

Abstract: The paper aims to study cryopreservation techniques of Trichogramma pintoi Voegele, and to provide references for large-scale breeding. We utilized different treatments such as water, 0.5%, 1.0%, 1.5% of saline water and cryopreservation directly, studied cryopreservation techniques of Trichogramma pintoi Voegele. The results showed that: compared with contrast, the treatments with 0.5%, 1.0%, 1.5% of saline water could improve eclosion rate and reduce eclosion aberration rate; the fecundity per female treated by each concentration of saline water had no significant difference compared with the contrast within 45 days, indicating that Trichogramma pintoi Voegele treated by each concentration of saline water could maintain fecundity per female within 45 days; the female-male rate increased after cryopreservation for 90 days, and female-male rate became imbalanced after cryopreservation for 75 days. The research also showed that: the cryopreservation time increase was against the development of richogramma pintoi Voegele, whereas the treatments with 0.5%, 1.0%, and 1.5% saline water could reduce negative effects, which were favorable factors on eclosion rate, eclosion aberration rate, female life span, fecundity per female and female-male rate.