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中国农学通报

中国农学通报 ›› 2020, Vol. 36 ›› Issue (8): 91-97.doi: 10.11924/j.issn.1000-6850.casb18110008

所属专题： 畜牧兽医； 园艺

• 畜牧·动物医学·蚕·蜂 • 上一篇    下一篇

山东地区乳房炎和非乳房炎牛奶中金黄色葡萄球菌的分离鉴定与耐药分析

张青青1,2, 刘娜1, 王娟1, 赵格1, 赵建梅1, 李月华1, 黄秀梅1, 盖文燕1, 曲志娜1(), 刘焕奇2()   

  1. 1 中国动物卫生与流行病学中心,山东青岛 266032
    2 青岛农业大学动物医学院,山东青岛 266109
  • 收稿日期:2018-11-16 修回日期:2019-02-07 出版日期:2020-03-15 发布日期:2020-03-10
  • 通讯作者: 曲志娜,刘焕奇
  • 作者简介:张青青,女,1993年出生,山东平度人,硕士,研究方向：临床兽医学。通信地址：266032 山东省青岛市市北区南京路369号 中国动物卫生与流行病学中心,E-mail：18363974857@163.com。
  • 基金资助:
    山东省现代农业产业技术体系创新团队项目(SDAI09-03);国家重点研发计划专项"畜禽养殖用药新技术研究"(2017YFD0501406)

Isolation, Identification and Drug Resistance of Staphylococcus aureus from Mastitis and Non-mastitis Milk in Shandong

Zhang Qingqing1,2, Liu Na1, Wang Juan1, Zhao Ge1, Zhao Jianmei1, Li Yuehua1, Huang Xiumei1, Gai Wenyan1, Qu Zhina1(), Liu Huanqi2()   

  1. 1 China Animal Health and Epidemiology Center, Shandong Qingdao 266032
    2 Qingdao Agricultural University, College of Veterinary Medicine, Shandong Qingdao 266109
  • Received:2018-11-16 Revised:2019-02-07 Online:2020-03-15 Published:2020-03-10
  • Contact: Zhina Qu,Huanqi Liu

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摘要：

为了解山东地区不同类型牛奶中金黄色葡萄球菌流行情况和耐药特征,对37个牧场的乳房炎和非乳房炎奶样进行细菌分离鉴定及MIC测定试验。采用常规方法与PCR技术进行金黄色葡萄球菌的分离鉴定,微量肉汤稀释法进行MIC测定,卡方检验进行数据差异显著性分析。①共分离到150株金黄色葡萄球菌,检出率为11.26%(150/1332),且乳房炎奶样显著高于非乳房炎(P=0.009);②12种药物的MIC与EUCAST分布趋势大致相同;③分离菌株对青霉素(91.38%)耐药最严重,且乳房炎/非乳房炎奶样差异显著(P=0.047);④116株分离菌株中多重耐药(≥3)菌株为34(29.31%)株,乳房炎/非乳房炎奶样差异不显著(P=0.611);⑤威海对6种药物的耐药率在60%以上,其他地区仅对1~2种药物的耐药率在50%以上。乳房炎奶样的金黄色葡萄球菌检出率显著高于非乳房炎;分离菌株对青霉素严重耐药且乳房炎奶样多重耐药问题突出;不同地区均呈现不同程度的耐药。

关键词: 乳房炎, 非乳房炎, 金黄色葡萄球菌, 分离鉴定, 耐药

Abstract:

To understand the prevalence and drug resistance of Staphylococcus aureus in different types of milk in Shandong, bacterial isolation, identification and minimal inhibitory concentration (MIC) test were carried out on mastitis and non-mastitis milk samples from 37 farms. The isolation and identification of Staphylococcus aureus were carried by conventional method and PCR technology, the MIC test was conducted with broth micro dilution methods and the data were analyzed by chi-square test. ①A total of 150 strains of Staphylococcus aureus were isolated with the detection rate of 11.26% (150/1332), and the milk samples of mastitis was significantly higher than that of non-mastitis (P=0.009). ②The distribution tend of MIC of 12 drugs was roughly the same as that of EUCAST. ③The isolated strains were the most resistant to penicillin (91.38%), and there was a significant difference in mastitis and non-mastitis milk samples (P=0.047). ④In 116 separation strains, there were 34 (29.31%) strains of multiple resistance (≥3) and there was no significant difference in mastitis and non-mastitis milk (P=0.611). ⑤The resistance rate of six drugs in Weihai was more than 60% and the resistance rate of only one or two drugs in other regions was above 50%. The detection rate of Staphylococcus aureus in mastitis milk samples was significantly higher than that in non-mastitis milk samples. The isolated strain was highly resistant to penicillin and the problem of multiple drug resistance of mastitis milk samples was serious. Different regions showed different levels of drug resistance.

Key words: mastitis, non-mastitis, Staphylococcus aureus, isolation and identification, drug resistance

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