中国农学通报 ›› 2019, Vol. 35 ›› Issue (16): 69-76.doi: 10.11924/j.issn.1000-6850.casb19010082
所属专题: 园艺
王焕丽, 刘大军, 冯国军, 刘 畅, 杨晓旭, 范有君, 闫志山
收稿日期:
2019-01-16
修回日期:
2019-05-13
接受日期:
2019-04-24
出版日期:
2019-06-04
发布日期:
2019-06-04
通讯作者:
刘大军
基金资助:
Received:
2019-01-16
Revised:
2019-05-13
Accepted:
2019-04-24
Online:
2019-06-04
Published:
2019-06-04
摘要: 菜豆分子育种及功能基因组学分析需要高效稳定的再生体系和遗传转化体系。归纳了近年来国内外菜豆再生体系及遗传转化体系的研究进展。首先阐述了菜豆再生方法及研究现状,其中包括基于器官发生和体细胞胚发生不同途径菜豆再生体系的建立和相关的影响因素。其次概括了农杆菌介导法在菜豆遗传转化方面的研究进展,并分析了影响农杆菌介导遗传转化效率的主要因素,包括受体外植体类型和外植体损伤、农杆菌菌株及菌液浓度、共培养条件(时间、温度、光照时间)、乙酰丁香醇浓度等。然后叙述了有关基因枪介导菜豆遗传转化的研究进展。最后对目前菜豆再生体系和遗传转化体系存在的难题进行了总结,以期为建立菜豆的高效离体再生体系和遗传转化体系提供重要参考。
中图分类号:
王焕丽,刘大军,冯国军,刘 畅,杨晓旭,范有君,闫志山. 菜豆再生体系及遗传转化体系研究进展[J]. 中国农学通报, 2019, 35(16): 69-76.
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