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中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (22): 47-52.doi: 10.11924/j.issn.1000-6850.casb19020060

所属专题： 园艺

• 生物技术科学 • 上一篇    下一篇

基于SSR、InDel分子标记的红甜菜遗传多样性分析

刘蕊1, 刘乃新2, 吴玉梅2, 吴则东2, 兴旺2, 王秋红2   

  1. 1.青海大学农牧学院;2.黑龙江大学农作物研究院
  • 收稿日期:2019-02-26 修回日期:2019-07-08 接受日期:2019-04-24 出版日期:2019-08-13 发布日期:2019-08-13
  • 通讯作者: 吴玉梅
  • 基金资助:
    国家现代农业产业技术体系（糖料）建设专项“质量安全与营养品质评价”(CARS-17-0503)；国家农产品质量安全风险评估专项经费“枸杞 人参百合糖料中典型污染物残留及安全控制点风险评估工作”(GJFP2019020)；农业行业标准项目“甜菜种子生产技术规程”。

Genetic Diversity Analysis of Red Beet: Based on SSR and Indel Molecular Markers

  • Received:2019-02-26 Revised:2019-07-08 Accepted:2019-04-24 Online:2019-08-13 Published:2019-08-13

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摘要： 本研究旨在了解21份国内外红甜菜品种的遗传多样性及其演化地位。利用Indel和SSR分子标记，对21份红甜菜遗传多样性研究。结果表明，InDel标记共得到25个基因位点，24个位点具有多态性，Shannon’s信息指数平均值为0.583，Nei’s基因多样性指数平均值为0.370，等位基因K为2~4个，平均2.4个，平均观测杂合度为0.235，期望杂合度0.398，多态信息含量PIC值为0.330；SSR标记共扩增出28个基因位点，全部具有多态性，平均每个标记扩增出3.5个多态性基因位点，Shannon’s 信息指数平均值为0.926，Nei’s基因多样性指数平均值为0.548，等位基因K为2~7个，平均4.625个，平均观测杂合度0.399，期望杂合度0.587，多态信息含量PIC值为0.523。本研究发现红甜菜品种大多因亲缘关系较近，因此在选育新品种时，建议尽量选择遗传距离较远的品种作为亲本。

关键词: 紫花苜蓿, 紫花苜蓿, CpFATB, 生理功能, 抗寒性

Abstract: The paper aim to understand the genetic diversity and evolutionary status of 21 varieties of red beet.Genetic diversity of 21 red beets was studied by InDel and SSR markers.The results showed that the molecular markers of InDel had 25 gene loci, 24 of which were polymorphic. The Shannon''s information index average was 0.583, the Nei''s gene diversity index average was 0.370, the allele K was 2~4, and the average was 2.4. The average observed heterozygosity was 0.235, the expected heterozygosity was 0.398, and the PIC of polymorphic information content was 0.330.The average values of Shannon''s information index were 0.926, the average values of Nei''s gene diversity index were 0.548, the average values of alleles K were 2~7, with an average of 4.625. The average observed heterozygosity was 0.399, the expected heterozygosity was 0.587, and the PIC value of polymorphic information content was 0.523.In this study, it was found that most red beets were closely related to each other. Therefore, when selecting new varieties, it is suggested to select the varieties with longer genetic distance as parents.