产纤维素酶菌系的添加对厌氧干发酵产沼气的影响

doi:10.11924/j.issn.1000-6850.casb2021-0009

摘要/Abstract

摘要：

为了提高农作物秸秆和畜禽粪便的利用率,通过在沼气厌氧发酵系统中添加降解纤维素类复合菌系,以提高产气性能。试验以玉米秸秆和牛粪为原料,在发酵温度为37℃,固体物浓度为20%,接种物含量为30%,粪草比为1:2的发酵条件下中接入1%的复合菌系进行厌氧干发酵产沼气的研究。试验结果表明由Bacillus siamensis和Bacillus subtilis subsp. Stercoris构成的复合菌系使沼气产量提高25%,甲烷产量提高55%,原料产气分别为86.4 mL/gTS和111.4 mL/gVS。试验为纤维素类生物质能的利用提供了一定的参考价值。

关键词: 玉米秸秆, 牛粪, 干发酵, 复合菌系, 池容产气率

Abstract:

In order to improve the utilization rate of crop straws and livestock manure, a cellulose-degrading compound flora is added to the biogas anaerobic fermentation system to improve gas production performance. The experiment used corn stalk and cow dung as raw materials. The fermentation condition was: 37℃ of fermentation temperature, 20% of total solid concentration, 30% of inoculum content, 1:2 of manure-to-grass ratio, and 1% of the compound flora was added to anaerobic dry fermentation to produce biogas. The results show that the compound flora consisting of Bacillus siamensis and Bacillus subtilis subsp. Stercoris could increase biogas production by 25% and methane production by 55%, and gas production rate of raw materials is 86.4 mL/gTS and 111.4 mL/gVS, respectively. The experiment could provide certain reference value for the utilization of biomass energy containing cellulose.

Key words: corn straw, cow manure, dry fermentation, compound flora, volumetric biogas yield

中图分类号:

S216.2

张陈, 闫红心, 纪栋, 魏志豪, 姚志松, CAMARA ZOUMANA, 苏有勇. 产纤维素酶菌系的添加对厌氧干发酵产沼气的影响[J]. 中国农学通报, 2021, 37(17): 144-150.

Zhang Chen, Yan Hongxin, Ji Dong, Wei Zhihao, Yao Zhisong, CAMARA ZOUMANA, Su Youyong. Effect of Adding Cellulase-Producing Compound Flora on Biogas Production by Anaerobic Dry Fermentation[J]. Chinese Agricultural Science Bulletin, 2021, 37(17): 144-150.

图/表 11

参考文献 36

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原料	TS/%	VS/%	木质素/%	半纤维素/%	纤维素/%
接种物	13.87	8.81	1.91	0.50	1.19
玉米秸秆	90.18	81.95	10.18	18.77	35.95
牛粪	55.98	37.49	12.49	7.62	7.77

原料	TS/%	VS/%	木质素/%	半纤维素/%	纤维素/%
接种物	13.87	8.81	1.91	0.50	1.19
玉米秸秆	90.18	81.95	10.18	18.77	35.95
牛粪	55.98	37.49	12.49	7.62	7.77

组别	粪草比	发酵浓度/%	粪草TS添加量/g	粪草实际添加量/g	发酵总质量/g
空白组	-	-	-	-	200
对照组	1:2	20	11.26:22.53	25.8:25.0	200
试验组	1:2	20	11.26:22.53	25.8:25.0	200

组别	粪草比	发酵浓度/%	粪草TS添加量/g	粪草实际添加量/g	发酵总质量/g
空白组	-	-	-	-	200
对照组	1:2	20	11.26:22.53	25.8:25.0	200
试验组	1:2	20	11.26:22.53	25.8:25.0	200

菌株编号	鉴定结果
ZC-1	Bacillus tequilensis
ZC-3	Bacillus velezensis
NF-3	Bacillus siamensis
NF-4	Bacillus subtilis subsp. Stercoris
YJ-2	Pantoea dispersa
YJ-5	Cronobacter dublinensis subsp. lausannensis