不同发育阶段‘文紫椒1号’果色色素的比例和组织定位

doi:10.11924/j.issn.1000-6850.casb2021-0493

中国农学通报 ›› 2022, Vol. 38 ›› Issue (10): 38-45.doi: 10.11924/j.issn.1000-6850.casb2021-0493

所属专题：生物技术；园艺

不同发育阶段‘文紫椒1号’果色色素的比例和组织定位

屈用函¹(), 袁恩平², 张雪廷², 王绍祥², 王灿², 赵水灵², 李罡², 顾凡³, 张洪玲³, 赵昶灵³(), 李桂琼³(), 陈杞临³

¹文山壮族苗族自治州农业科学院生态环境资源研究所,云南文山 663000
²文山壮族苗族自治州农业科学院蔬菜研究所,云南文山 663000
³云南农业大学农学与生物技术学院,昆明 650201

收稿日期:2021-05-11 修回日期:2022-02-10 出版日期:2022-04-05 发布日期:2022-05-23
通讯作者: 赵昶灵,李桂琼
作者简介:屈用函,男,1982年出生,云南文山人,高级农艺师,主要从事辣椒遗传育种研究。通信地址：663000 文山壮族苗族自治州农业科学院生态环境资源研究所,Tel：0876-3037546,E-mail： xiaoqyh@163.com。
基金资助:
文山壮族苗族自治州农业科学院资助项目“丘北辣椒紫色果实花色苷合成相关基因对花色苷合成的差异性贡献”(2019WF006);“丘北辣椒紫色果实花色苷结构基因的发育依赖表达模式及其与花色苷含量相关性”(HZ2019-01);“丘北辣椒紫色果实花色苷转录因子基因的发育依赖表达模式及其与花色苷含量相关性”(HZ2019-01-A1)

Ratios and Tissue Locations of Fruit Color Pigments of Capsicum annuum ‘Wenzijiao No.1’ at Different Development Stages

QU Yonghan¹(), YUAN Enping², ZHANG Xueting², WANG Shaoxiang², WANG Can², ZHAO Shuiling², LI Gang², GU Fan³, ZHANG Hongling³, ZHAO Changling³(), LI Guiqiong³(), CHEN Qilin³

¹Institute of Ecological Environment and Resources, Academy of Agricultural Sciences of Wenshan Zhuang and Miao Autonomous Prefecture, Wenshan, Yunnan 663000
²Institute of Vegetable Crops, Academy of Agricultural Sciences of Wenshan Zhuang and Miao Autonomous Prefecture, Wenshan, Yunnan 663000
³College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201

Received:2021-05-11 Revised:2022-02-10 Online:2022-04-05 Published:2022-05-23
Contact: ZHAO Changling,LI Guiqiong

摘要/Abstract

摘要：

研究旨在阐明‘文紫椒1号’果实在不同发育阶段的色素比例和组织定位,为丘北辣椒在观赏园艺中的应用和观赏型新奇果色新品种的选育提供参考。以种植于云南文山州砚山县的‘文紫椒1号’果实为试材,将果实分为5个发育阶段,用分光光度法检测了果实的叶绿素含量([Chl])、类胡萝卜素含量([Car])、总黄酮类含量(TFC)、总花色苷含量(TAC)和辣椒红素含量([Cap]),并分析其配比和相关性,同时用徒手切片法检测了色素的组织定位。结果表明,在发育过程中,‘文紫椒1号’果实[Chla]/[Chlb]的最大值出现在发育阶段3,[Chl(a+b)]/[Car]、[Chl(a+b)]/TFC和[Chl(a+b)]/TAC的最大值均出现在阶段2,而[Chl(a+b)]/[Cap]、TFC/TAC、TFC/[Cap]和TAC/[Cap]的最大值均出现在阶段1。[Chla]、[Chlb]、[Chl(a+b)]、[Car]、TFC、TAC和[Cap]之间的相关性不尽一致,其中,TAC与[Chl(a+b)]、TFC之间均呈不显著正相关,而与[Car]之间呈不显著负相关,[Cap]则与[Chl(a+b)]、[Car]、TFC和TAC之间分别呈显著负相关、极显著正相关、不显著正相关和不显著负相关。果实色素主要定位在中果皮细胞中,细胞的色相和色度变化趋势与果色的高度一致。所以,‘文紫椒1号’的果色源于各发育阶段果皮细胞中叶绿素、类胡萝卜素（含辣椒红素）和黄酮类（含花色苷）色素的比例差异。

关键词: ‘文紫椒1号’, 发育阶段, 果色色素, 比例, 组织定位

Abstract:

This research aims to elucidate the ratios and tissue locations of the pigments of Capsicum annuum ‘Wenzijiao No.1’ fruits at different development stages, and provide reference for the application of Qiubei chili in ornamental horticulture and the breeding of new ornamental chili cultivars with novel fruit pigmentation. The fruits of the C. annuum ‘Wenzijiao No.1’ planted in Yanshan County of Wenshan Zhuang and Miao Autonomous Prefecture in Yunnan Province were used as the test materials, and the fruits were divided into five development stages, the chlorophyll content ([Chl]), carotenoid content ([Car]), total flavonoid content (TFC), total anthocyanin content (TAC) and capsanthin content ([Cap]) of the fruits were determined by using spectrophotometry, and their ratios and correlations were analyzed. Simultaneously, the tissue locations of the pigments were determined by using freehand slicing. The results indicated that, during the development process, the maximum value of [Chla]/[Chlb] of C. annuum ‘Wenzijiao No.1’ fruits appeared at the third development stage (Stage 3), the maximum values of [Chl(a+b)]/[Car], [Chl(a+b)]/TFC and [Chl(a+b)]/TAC all at Stage 2, whereas that of [Chl(a+b)]/[Cap], TFC/TAC, TFC/[Cap] and TAC/[Cap] all at Stage 1. The correlations among [Chla], [Chlb], [Chl(a+b)], [Car], TFC, TAC and [Cap] were not consistent, and among them, TAC was positively correlated with [Chl(a+b)] and TFC both at non-significant levels, but negatively correlated with [Car] at a non-significant level, whereas there was a significantly negative correlation between [Cap] and [Chl(a+b)], an extremely and significantly positive one between [Cap] and [Car], a non-significantly positive one between [Cap] and TFC, and a non-significantly negative one between [Cap] and TAC. Simultaneously, the fruit pigments were mainly located in the mesocarp cells, and the changing trend of the hue and chroma of the cells was highly consistent with that of the fruit color. So, the pigmentation of C. annuum ‘Wenzijiao No.1’ fruits results from the differentiated ratios of chlorophyll, carotenoid (including capsanthin) and flavonid (including anthocyanin) pigments in the pericarp cells at different development stages.

Key words: Capsicum annuum ‘Wenzijiao No.1’, development stage, fruit color pigment, ratio, tissue location

中图分类号:

S641.3

屈用函, 袁恩平, 张雪廷, 王绍祥, 王灿, 赵水灵, 李罡, 顾凡, 张洪玲, 赵昶灵, 李桂琼, 陈杞临. 不同发育阶段‘文紫椒1号’果色色素的比例和组织定位[J]. 中国农学通报, 2022, 38(10): 38-45.

QU Yonghan, YUAN Enping, ZHANG Xueting, WANG Shaoxiang, WANG Can, ZHAO Shuiling, LI Gang, GU Fan, ZHANG Hongling, ZHAO Changling, LI Guiqiong, CHEN Qilin. Ratios and Tissue Locations of Fruit Color Pigments of Capsicum annuum ‘Wenzijiao No.1’ at Different Development Stages[J]. Chinese Agricultural Science Bulletin, 2022, 38(10): 38-45.

图/表 8

图1. ‘文紫椒1号’果实在不同发育阶段的叶绿素a/b和[叶绿素(a+b)]/[类胡萝卜素]

图2. ‘文紫椒1号’果实在不同发育阶段的色素含量

图3. ‘文紫椒1号’果实在不同发育阶段的[叶绿素]/[总黄酮类]

图4. ‘文紫椒1号’果实在不同发育阶段的[总叶绿素]/[总花色苷]和[叶绿素]/[辣椒红素]

图5. ‘文紫椒1号’果实在不同发育阶段的[总黄酮类]/[总花色苷]和[总黄酮类]/[辣椒红素]

图6. ‘文紫椒1号’果实在不同发育阶段的[总花色苷]/[辣椒红素]

图7. 不同发育阶段‘文紫椒1号’果实果皮中色素的组织定位和积累量数字1、2、3、4和5对应果实被划分为5个发育阶段,100×

	[Chla]	[Chlb]	[Chl(a+b)]	[Car]	TFC	TAC	[Cap]
[Chla]	1	0.984^**	0.999^**	-0.931^*	0.111	0.441	-0.897^*
[Chlb]		1	0.990^**	-0.960^**	-0.238	0.335	-0.934^*
[Chl(a+b)]			1	-0.939^*	-0.135	0.422	-0.907^*
[Car]				1	0.458	-0.142	0.995^**
TFC					1	0.768	0.535
TAC						1	-0.042
[Cap]							1

表1. ‘文紫椒1号’果实在发育过程中各色素含量间的Pearson相关系数

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不同发育阶段‘文紫椒1号’果色色素的比例和组织定位

Ratios and Tissue Locations of Fruit Color Pigments of Capsicum annuum ‘Wenzijiao No.1’ at Different Development Stages

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