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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (27): 95-102.doi: 10.11924/j.issn.1000-6850.casb2021-0624

• 生物科学 • 上一篇    下一篇

水稻ENOD93基因家族的全基因组鉴定与生物信息学分析

李固梓(), 刘群恩, 陈代波, 于萍()   

  1. 中国水稻研究所/浙江省超级稻研究重点实验室，杭州 311401
  • 收稿日期:2021-06-28 修回日期:2021-09-13 出版日期:2023-09-25 发布日期:2023-09-22
  • 通讯作者: 于萍，女，1982年出生，内蒙古兴安盟人，副研究员，博士，主要从事水稻鉴定评价与种质创新。通信地址：311401 浙江省杭州富阳区水稻所路28号 中国水稻研究所，Tel：0571-63370272，E-mail：pingping367@163.com。
  • 作者简介:

    李固梓，女，2000年出生，浙江温州人，硕士研究生在读，研究方向：水稻鉴定评价与种质创新。通信地址：311401 浙江省杭州富阳区水稻所路28号 中国水稻研究所，Tel：0571-63370218，E-mail：。

  • 基金资助:
    国家自然科学基金“OsENOD93-1调控水稻氮利用率的机理研究”(32100224); 浙江省科技计划项目“水稻遗传资源数字化利用技术体系构建”(2021C02056)

Genome-wide Identification and Bioinformatic Analysis of ENOD93 Gene Family in Rice

LI Guzi(), LIU Qun’en, CHEN Daibo, YU Ping()   

  1. Key Laboratory for Zhejiang Super Rice Research/China National Rice Research Institute, Hangzhou 311401
  • Received:2021-06-28 Revised:2021-09-13 Published-:2023-09-25 Online:2023-09-22

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摘要：

旨在为非豆科植物中早期结瘤素蛋白ENOD93的功能研究提供参考和依据。利用生物信息学方法对水稻中的ENOD93基因家族成员进行鉴定，并对其成员的理化性质、染色体定位、基因结构、蛋白结构、表达谱、进化关系进行分析。结果表明，水稻ENOD93基因家族有7个成员，分布在第2和第6染色体上，基因结构均较简单，而且大部分ENOD93基因在保守结构域和保守基序的分布和排列上具有高度的相似性。RNA-Seq数据分析结果表明，水稻ENOD93基因在雌蕊、种子和胚中高表达，部分基因的表达量还受到逆境胁迫的诱导。利用包含单、双子叶植物的9个物种进行的系统进化关系分析，将31个ENOD93基因家族成员分为4个不同的类群。水稻ENOD93基因在不同组织和不同发育时期的表达存在差异，部分基因受胁迫诱导表达，表明该家族基因参与植物众多组织的发育过程且在逆境胁迫响应中具有重要作用。

关键词: 水稻, ENOD93基因家族, 基因鉴定, 生物信息学, 逆境胁迫

Abstract:

The aim of this study was to provide reference for the functional study of early nodulation protein ENOD93 in non-legumes. In this study, bioinformatics method was used to identify rice ENOD93 gene family. The physical and chemical properties, chromosome location, gene structure, protein structure, expression spectrum and evolutionary relationship of the members were analyzed. The results showed that there were 7 members of rice ENOD93 gene family, which were distributed on chromosomes 2 and 6, and the gene structure was relatively simple. Moreover, most ENOD93 genes are highly similar in the distribution and arrangement of conserved domain and motif. The results of RNA-Seq data analysis showed that ENOD93 gene family was highly expressed in pistils, seeds and embryos, and the expression level of some gene was induced by stress. Based on the phylogenetic analysis of nine monocotyledonous and dicotyledonous species, 31 ENOD93 gene family members were divided into four distinct groups. The expression of ENOD93 gene in rice was different in different tissues and at different developmental stages, and some genes were induced by stress, suggesting that ENOD93gene family was involved in the development process of many plant tissues and played an important role in the response to stress.

Key words: rice, ENOD93 gene family, gene identification, bioinformatics, adversity stress