甘草产香内生真菌RP2的初步鉴定及挥发性成分分析

doi:10.11924/j.issn.1000-6850.casb2021-0668

中国农学通报 ›› 2022, Vol. 38 ›› Issue (12): 138-145.doi: 10.11924/j.issn.1000-6850.casb2021-0668

所属专题：生物技术

• 食品·营养·检测·安全 • 上一篇下一篇

甘草产香内生真菌RP2的初步鉴定及挥发性成分分析

尤梦瑶¹^,²(), 闫佳佳¹^,², 万璐¹^,², 张赫¹^,², 郑春英¹^,²()

¹黑龙江大学/农业微生物技术教育部工程研究中心,哈尔滨 150500
²黑龙江大学生命科学学院/黑龙江省普通高校微生物重点实验室,哈尔滨 150080

收稿日期:2021-07-06 修回日期:2021-10-13 出版日期:2022-04-25 发布日期:2022-05-18
通讯作者: 郑春英
作者简介:尤梦瑶,女,1997年出生,黑龙江牡丹江人,硕士研究生,研究方向：食品和药物生物活性挖掘及研发。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学生命科学学院,Tel：0451-86608586,E-mail： youmengyao4u@163.com。
基金资助:
黑龙江大学校企合作横向项目“一株产原儿茶酸的枯草芽孢杆菌”(20170223)

An Aroma-producing Endophytic Fungus RP2 from Glycyrrhiza uralensis: Preliminary Identification and Volatile Components' Analysis

YOU Mengyao¹^,²(), YAN Jiajia¹^,², WAN Lu¹^,², ZHANG He¹^,², ZHENG Chunying¹^,²()

¹Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500
²School of Life Sciences, Heilongjiang University/Key Laboratory of Microbiology, College of Heilongjiang Province, Harbin 150080

Received:2021-07-06 Revised:2021-10-13 Online:2022-04-25 Published:2022-05-18
Contact: ZHENG Chunying

摘要/Abstract

摘要：

为了开发甘草内生真菌RP2次生代谢产物的资源,丰富挥发性香味成分的获取途径,以甘草内生真菌RP2为研究对象,采用形态学观察及ITS序列分析法对其进行菌种鉴定,通过气相色谱-质谱联用技术(GC-MS)对甘草内生真菌RP2挥发性成分进行分析。甘草内生真菌RP2经鉴定为尖孢镰刀菌(Fusarium oxysporum)。在甘草内生真菌RP2发酵液中分离并鉴定出11种挥发性成分;在甘草内生真菌RP2菌丝体中分离并鉴定了6种挥发性成分。甘草内生真菌RP2挥发性成分中含有多种现有工业生产中广泛应用的挥发性香味成分,如2-苯基乙醇等。本研究对开发利用甘草内生真菌RP2具有重要意义。

关键词: 甘草内生真菌RP2, 形态学观察, ITS序列分析, 气相色谱-质谱联用, 挥发性成分

Abstract:

The study aims to explore secondary metabolite resources of endophytic fungus RP2 which is isolated from Glycyrrhiza uralensis and increase the accessing ways for volatile aroma components. Endophytic fungus RP2 was chosen as the object and identified by morphological observation and ITS sequence analysis, and its volatile components were analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that endophytic fungus RP2 was identified as Fusarium oxysporum. 11 volatile components were isolated and identified from the RP2 fermentation liquid, and 6 components were isolated and identified from RP2 mycelium. RP2 can produce a variety of volatile aroma components that can be widely used in current industrial production, such as 2-phenylethanol. This study is of great significance for the development and utilization of RP2.

Key words: endophytic fungus RP2 from Glycyrrhiza uralensis, morphological observation, ITS sequence analysis, GC-MS, volatile components

中图分类号:

S682.31

尤梦瑶, 闫佳佳, 万璐, 张赫, 郑春英. 甘草产香内生真菌RP2的初步鉴定及挥发性成分分析[J]. 中国农学通报, 2022, 38(12): 138-145.

YOU Mengyao, YAN Jiajia, WAN Lu, ZHANG He, ZHENG Chunying. An Aroma-producing Endophytic Fungus RP2 from Glycyrrhiza uralensis: Preliminary Identification and Volatile Components' Analysis[J]. Chinese Agricultural Science Bulletin, 2022, 38(12): 138-145.

图/表 7

参考文献 16

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序号	保留时间/min	分子式	化合物名称	相对含量/%
1	10.34	C₆H₈O₄	3,5-二羟基-6-甲基-2,3-二氢吡喃-4-酮	1.80
2	13.22	C₆H₆O₃	5-(羟甲基)呋喃-2-甲醛	1.00
3	18.51	C₁₅H₃₂	2,6,11-三甲基十二烷	0.42
4	31.98	C₁₀H₁₆O₂	(R)-6-戊基-5,6-二氢吡喃-2-酮	0.36
5	83.57	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	49.12
6	205.04	C₄₂H₆₃O₄P	磷酸三（2,4-二叔丁基苯基）酯	0.97

序号	保留时间/min	分子式	化合物名称	相对含量/%
1	10.34	C₆H₈O₄	3,5-二羟基-6-甲基-2,3-二氢吡喃-4-酮	1.80
2	13.22	C₆H₆O₃	5-(羟甲基)呋喃-2-甲醛	1.00
3	18.51	C₁₅H₃₂	2,6,11-三甲基十二烷	0.42
4	31.98	C₁₀H₁₆O₂	(R)-6-戊基-5,6-二氢吡喃-2-酮	0.36
5	83.57	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	49.12
6	205.04	C₄₂H₆₃O₄P	磷酸三（2,4-二叔丁基苯基）酯	0.97

序号	保留时间/min	分子式	化合物名称	相对含量/%
1	9.53	C₈H₁₀O	2-苯基乙醇	3.00
2	17.49	C₈H₁₀O₂	1-苯基乙烷-1,2-二醇	1.60
3	24.51	C₆H₆N₂O	尼克酰胺	0.54
4	27.15	C₈H₁₀O₂	对羟基苯乙酸	1.04
5	32.70	C₁₄H₄₂O₇Si₇	2,2,4,4,6,6,8,8,10,10,12,12,14,14-十四甲基-1,3,5,7,9,11,13-七氧杂-2,4,6,8,10,12,14-七硅杂环十四烷	0.23
6	36.54	C₁₄H₂₂O	2,4-二-叔-丁基苯酚	0.33
7	48.49	C₈H₈O₃	2-(4-羟基苯基)乙酸	0.96
8	77.20	C₁₀H₉NO₂	2-(1H-吲哚-1-基)乙酸	0.84
9	83.37	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	28.00
10	116.12	C₂₁H₃₀O₂	2-((1R,6R)-3-甲基-6-(丙-1-烯-2-基)环己-2-烯基)-5-戊基苯-1,3-二酚	0.69
11	157.61	C₂₂H₄₃NO	芥酰胺	1.31

序号	保留时间/min	分子式	化合物名称	相对含量/%
1	9.53	C₈H₁₀O	2-苯基乙醇	3.00
2	17.49	C₈H₁₀O₂	1-苯基乙烷-1,2-二醇	1.60
3	24.51	C₆H₆N₂O	尼克酰胺	0.54
4	27.15	C₈H₁₀O₂	对羟基苯乙酸	1.04
5	32.70	C₁₄H₄₂O₇Si₇	2,2,4,4,6,6,8,8,10,10,12,12,14,14-十四甲基-1,3,5,7,9,11,13-七氧杂-2,4,6,8,10,12,14-七硅杂环十四烷	0.23
6	36.54	C₁₄H₂₂O	2,4-二-叔-丁基苯酚	0.33
7	48.49	C₈H₈O₃	2-(4-羟基苯基)乙酸	0.96
8	77.20	C₁₀H₉NO₂	2-(1H-吲哚-1-基)乙酸	0.84
9	83.37	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	28.00
10	116.12	C₂₁H₃₀O₂	2-((1R,6R)-3-甲基-6-(丙-1-烯-2-基)环己-2-烯基)-5-戊基苯-1,3-二酚	0.69
11	157.61	C₂₂H₄₃NO	芥酰胺	1.31

序号	保留时间/min	分子式	化合物名称	相对含量/%
1	13.99	C₈H₈O₂	2-羟基-1-苯基乙酮	0.67
2	19.53	C₈H₁₀O₂	苯乙二醇	34.42
3	62.89	C₁₇H₂₄O₄	邻苯二甲酸5-甲基-2-乙基酯	9.54
4	82.87	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	5.50
5	116.18	C₂₁H₃₀O₂	2-((1R,6R)-3-甲基-6-(丙-1-烯-2-基)环己-2-烯基)-5-戊基苯-1,3-二酚	0.29
6	157.80	C₂₂H₄₃NO	芥酰胺	1.36

甘草产香内生真菌RP2的初步鉴定及挥发性成分分析

An Aroma-producing Endophytic Fungus RP2 from Glycyrrhiza uralensis: Preliminary Identification and Volatile Components' Analysis

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