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中国农学通报

中国农学通报 ›› 2021, Vol. 37 ›› Issue (35): 25-33.doi: 10.11924/j.issn.1000-6850.casb2021-0677

所属专题： 生物技术

• 生物科学 • 上一篇    下一篇

能源甜菜转录因子BvMYB44基因响应镉胁迫的表达特性及生物信息学分析

汪曼1,2(), 周婉婷1,2, 周翔1,2, 李思琪1,2, 王雪倩1,2, 王录红1,2, 李王胜1,2, 李佳佳1,2, 高卓1,2,3, 刘大丽1,2()   

  1. 1国家甜菜种质中期库,黑龙江大学,哈尔滨 150080
    2省高校甜菜遗传育种重点实验室/黑龙江大学现代农业与生态环境学院,黑龙江大学,哈尔滨 150080
    3黑龙江省普通高等学校分子生物学重点实验室/黑龙江大学生命科学学院,哈尔滨 150080
  • 收稿日期:2021-07-13 修回日期:2021-09-13 出版日期:2021-12-15 发布日期:2022-01-07
  • 通讯作者: 刘大丽
  • 作者简介:汪曼,女,1998年出生,湖北宜昌人,硕士研究生,研究方向：作物分子生物学。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号 黑龙江大学现代农业与生态环境学院,E-mail： 1046918586@qq.com。
  • 基金资助:
    黑龙江省自然科学基金项目“BvHIPP24基因在能源甜菜重金属镉污染生物修复中的分子机制研究”(LH2019C057);黑龙江省高校基本科研业务费黑龙江大学专项资金项目“能源甜菜BvMPT11基因的Cd污染生物修复应答机制研究”(KJCX201920);科技部财政部国家科技资源共享服务平台项目“国家作物种质资源库甜菜分库运行服务”(NCGRC-2021-017);农作物种质资源保护与利用专项”甜菜种质资源的收集、鉴定、编目、繁种与入库(圃)保存”(1921-026);黑龙江省普通本科高等学校青年创新人才培养计划“甜菜抗旱遗传资源评价及优异基因挖掘”(UNPYSCT-20200140)

Transcription Factor BvMYB44 Gene in Energy Beet: Expression Characteristics in Response to Cadmium Stress and Its Bioinformatics Analysis

Wang Man1,2(), Zhou Wanting1,2, Zhou Xiang1,2, Li Siqi1,2, Wang Xueqian1,2, Wang Luhong1,2, Li Wangsheng1,2, Li Jiajia1,2, Gao Zhuo1,2,3, Liu Dali1,2()   

  1. 1National Beet Medium-term Gene Bank, Heilongjiang University, Harbin 150080
    2Key Laboratory of Sugar Beet Genetics and Breeding of Heilongjiang Province/College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
    3 Key Laboratory of Molecular Biology of Heilongjiang Province/School of Life Sciences, Heilongjiang University, Harbin 150080
  • Received:2021-07-13 Revised:2021-09-13 Online:2021-12-15 Published:2022-01-07
  • Contact: Liu Dali

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摘要：

为了解析BvMYB44在能源甜菜重金属胁迫中的调控作用,本研究以‘780016B/12优’为材料,通过qRT-PCR研究BvMYB44响应镉胁迫的表达模式,并利用生物信息学分析其功能。研究表明,BvMYB44基因cDNA全长942 bp,编码313个氨基酸,蛋白分子量为33935.01 Da,理论等电点为7.57。BvMYB44定位于细胞核中,具有39个磷酸化位点,为仅有1个外显子的不稳定碱性亲水蛋白。BvMYB44为R2R3-MYB亚家族成员,与藜麦CqMYB44-like和菠菜SoMYB44-like的亲缘关系最近。在该基因启动子区预测到多个与非生物胁迫相关的顺式作用元件,推测其可能参与多种非生物胁迫响应。qRT-PCR表明,在0.5 mmol/L镉胁迫处理下,BvMYB44在能源甜菜叶和根中均有不同程度的显著上调表达。因此,可以推断BvMYB44与镉胁迫存在着一定的应答关系。本研究将为抗重金属能源甜菜的种质创新以及拓展植物修复提供候选基因和理论基础。

关键词: 能源甜菜, BvMYB44, 生物信息学, 镉胁迫, 表达分析

Abstract:

In order to analyze the regulatory role of BvMYB44 of energy beet under heavy metal stress, this study used ‘780016B/12You’ as material to study the expression pattern of BvMYB44 in response to cadmium stress by qRT-PCR, and analyzed its function by bioinformatics. The results show that the total length of cDNA of BvMYB44 gene is 942 bp, encoding 313 amino acids, the molecular weight of the protein is 33935.01 Da, and the theoretical isoelectric point is 7.57. BvMYB44 is an unstable alkaline hydrophilic protein with 39 phosphorylation sites located in the nucleus and only one exon. BvMYB44 is a member of the R2R3-MYB subfamily and is most closely related to CqMYB44-like from Chenopodium quinoa and SoMYB44-like from Spinacia oleracea. Several cis-acting elements related to abiotic stress are predicted in the promoter region of this gene, which is speculated to be involved in a variety of abiotic stress responses. The qRT-PCR shows that BvMYB44 has different levels of significantly up-regulated expression in energy beet leaves and roots under 0.5 mmol/L cadmium stress. Therefore, it can be inferred that BvMYB44 has a certain response relationship with cadmium stress. This study will provide candidate genes and a theoretical basis for germplasm innovation of heavy metal-resistant energy beet and expansion of plant remediation.

Key words: energy beet, BvMYB44, bioinformatics, cadmium stress, expression analysis

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