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中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (32): 86-91.doi: 10.11924/j.issn.1000-6850.casb2021-1117

所属专题： 生物技术； 园艺

• 植物保护 • 上一篇    下一篇

辽宁新宾人参根腐病病原真菌的分离与鉴定

刘坤1,2,3(), 孙文松1,2,3(), 沈宝宇1,2,3, 张天静1,2,3   

  1. 1辽宁省经济作物研究所,辽阳 111000
    2辽宁省农业科学院药用植物研究所,辽阳 111000
    3中国医学科学院药用植物研究所辽阳研究中心,辽阳 111000
  • 收稿日期:2021-11-25 修回日期:2022-01-20 出版日期:2022-11-15 发布日期:2022-11-09
  • 通讯作者: 孙文松
  • 作者简介:刘坤,男,1986年出生,辽宁北票人,助理研究员,硕士,研究方向：植物病害综合防控。通信地址：111000 辽宁省辽阳市白塔区胜利路65号,Tel：0419-3678900,E-mail： yuanteng1986@126.com。
  • 基金资助:
    国家现代农业产业技术体系“国家中药材产业技术体系辽阳综合试验站”(CARS-21-06);国家重点研发计划“人参锈腐病生态防控技术集成与示范”(2060302);辽宁省农科院基本科研业务专项“中药材育种与栽培学科建设”(2019DD144221);辽宁省农科院重大科研成果培育项目“人参等中药材绿色增效技术集成研究与示范”(2020CGPY0403)

Isolation and Identification of Pathogenic Fungi Causing Panax ginseng Root Rot in Xinbin of Liaoning

LIU Kun1,2,3(), SUN Wensong1,2,3(), SHEN Baoyu1,2,3, ZHANG Tianjing1,2,3   

  1. 1Liaoning Research Institute of Cash Crops, Liaoyang, Liaoning 111000
    2Institute of Medicinal Plants, Liaoning Academy of Agricultural Sciences, Liaoyang, Liaoning 111000
    3Liaoyang Research Center of IMPLAD, Liaoyang, Liaoning 111000
  • Received:2021-11-25 Revised:2022-01-20 Online:2022-11-15 Published:2022-11-09
  • Contact: SUN Wensong

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摘要：

分离和鉴定辽宁人参主产区根腐病致病真菌,为人参抗病育种、开展根腐病绿色防治提供理论支撑。本研究采用组织分离法,对辽宁新宾人参根腐病重发生地块2~5年生病株开展植物病原真菌的分离和纯化。采用琼脂培养法,对分离菌株进行致病性测定。结合形态学观察和rDNA-ITS、EF-1α序列分析,对菌株开展形态学和分子生物学鉴定。最终,分离得到2株菌落形态不同的致病真菌,编号为JS64-1和JS64-3,经鉴定,分别为腐皮镰刀菌(Fusarium solani)和尖孢镰刀菌(Fusarium oxysporum)。本研究采用琼脂培养法,开展人参根腐病致病性测定,操作简便,省时高效,结果准确,可为其他植物根部病害研究提供参考。

关键词: 人参根腐病, EF-1α, 腐皮镰刀菌, 尖孢镰刀菌, 琼脂培养法, 致病性测定

Abstract:

In order to isolate and identify pathogenic fungi of root rot disease in main ginseng producing areas of Liaoning Province, and provide theoretical support for subsequent ginseng disease resistance breeding and green control of root rot disease, by using tissue isolation technique and malt-agar method, the pathogens of ginseng root rot were isolated and purified and the pathogenicity was tested with 2-5 years old infected plants from serious disease areas of Xinbin. Combined with morphological observation, rDNA-ITS and EF-1α sequence analysis, morphological and molecular biological identification of the strains were carried out. Finally, 2 pathogenic fungi were isolated, with different colony morphology, named strain JS64-1 and strain JS64-3, which were identified as Fusarium solani and Fusarium oxysporum, respectively. The malt-agar method, which was used to determine the pathogenicity of ginseng root rot in this article, was convenient and time-saving, and the results were accurate and reliable. The study could lay a foundation for research on root diseases of other plants.

Key words: ginseng root rot, EF-1α, Fusarium solani, Fusarium oxysporum, malt-agar method, pathogenicity test

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