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中国农学通报 ›› 2016, Vol. 32 ›› Issue (34): 108-112.doi: 10.11924/j.issn.1000-6850.casb16060064

所属专题: 园艺

• 植物保护 农药 • 上一篇    下一篇

新疆核桃树腐烂病致病力室内测定方法的研究

尹永香1,刘应敏1,马 荣2,赵 丽2,何美茹2,蔡桂芳2   

  1. (1新疆农业大学林业研究所,乌鲁木齐 830052;2新疆农业大学林学与园艺学院,乌鲁木齐 830052)
  • 收稿日期:2016-06-13 修回日期:2016-11-23 接受日期:2016-08-22 出版日期:2016-12-15 发布日期:2016-12-15
  • 通讯作者: 马荣
  • 基金资助:
    新疆维吾尔自治区自然科学基金“新疆核桃树腐烂病病原菌致病性分化研究”(2014211B019)。

Laboratory Evaluation Method of Walnut Canker Pathogenicity

Yin Yongxiang1, Liu Yingmin1, Ma Rong2, Zhao Li2, He Meiru2, Cai Guifang2   

  1. (1Forestry Research Institute, Xinjiang Agricultural University, Urumqi 830052;2College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052)
  • Received:2016-06-13 Revised:2016-11-23 Accepted:2016-08-22 Online:2016-12-15 Published:2016-12-15

摘要: 为探索出快速、可靠及操作简便的新疆核桃树腐烂病室内致病力测定方法,通过不同伤口类型将强致病力菌株的菌饼、分生孢子悬浮液和菌悬液接种在‘温185’核桃品种3种枝龄的枝条上,观测4种不同温度处理后核桃树腐烂病的发病程度得出最适致病条件。结果表明:3年生枝龄的核桃腐烂病发病最严重,病斑长度达到8.5 cm;通过烫伤处理后利用菌饼接种的离体枝条病斑长度最大,为9.8 cm;最利于病原菌扩展的温度为25℃,病斑长度达到11 cm;烫伤后放置0、1、2、3、4天后接种,病斑长度扩展无显著性差异性。研究结果为核桃腐烂病室内致病力的快速测定提供理论依据。

关键词: 芸薹根肿菌, 芸薹根肿菌, 激素, 转化酶, 芥子油苷, 致病机制, 研究进展

Abstract: The purpose of this project was to establish a fast, reliable and easy method to determine the virulence of walnut canker. Mycelium, spore suspension and bacterial suspension were inoculated on 1, 2 and 3 years old branches of ‘Wen 185’ with different wound treatments, and then pathogenic severity was observed with 4 different temperature treatments and then the optimium disease condition was obtained. The results showed that the canker of 3 years old walnut was most serious and the lesions length was up to 8.5 cm; the most ideal inoculum and wound treatments were agar disk and scalding because the lesions length was up to 9.8 cm; 25℃ was the optimum temperature for pathogenic bacteria to grow and the lesions length was up to 11 cm, and there was no difference of the inoculation time after scalding for the growth of pathogenic bacteria. The research results provide theoretical basis for a rapid laboratory determining method of walnut canker pathogenicity.