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中国农学通报 ›› 2023, Vol. 39 ›› Issue (19): 58-64.doi: 10.11924/j.issn.1000-6850.casb2022-0394

• 林学·园艺·园林 • 上一篇    下一篇

干旱胁迫和复水对萱草的生理特性的影响

王洋1(), 于森淼2, 王旌扬3, 宋海龙1, 雷耀东1, 张海燕1, 刘波1()   

  1. 1 山东正元地质资源勘查有限责任公司地质环境研究院,济南 250000
    2 南水北调中线信息科技有限公司,北京 100038
    3 青岛农业大学园林与林学院,山东青岛 266000
  • 收稿日期:2022-05-13 修回日期:2022-12-12 出版日期:2023-07-05 发布日期:2023-07-03
  • 通讯作者: 刘波,男,1984年出生,山东新泰人,高级工程师,硕士,研究方向为环境地质。通信地址:250101 济南市高新区舜华路1117号科汇大厦南区3楼正元 山东正元地质资源勘查有限责任公司地质环境研究院,Tel:0531-66770889,E-mail:154532525@qq.com
  • 作者简介:

    王洋,女,1984年出生,高级工程师,硕士,研究方向为园林植物应用与生态。E-mail:

  • 基金资助:
    横向项目“露天矿绿色矿山建设中矿山地质环境治理植物适应性试验项目”(6602421063)

Effects of Drought Stress and Rehydration on Physiological Characteristics of Hemerocallis fulva

WANG Yang1(), YU Senmiao2, WANG Jingyang3, SONG Hailong1, LEI Yaodong1, ZHANG Haiyan1, LIU Bo1()   

  1. 1 Geological Environment Research Institute, Shandong Zhengyuan Geological Resources Exploration Co. Ltd., Jinan 250000
    2 Information Technology Co., Ltd of South-to-North Water Diversion Middle Route Project, Beijing 100038
    3 College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, Shandong 266000
  • Received:2022-05-13 Revised:2022-12-12 Online:2023-07-05 Published:2023-07-03

摘要:

以萱草(Hemerocallis fulva)为试材,采用盆栽控水模拟干旱及复水环境的方式,测定其叶片相对含水量、叶绿素含量、相对电导率、抗氧化物酶的活性及渗透调节物质的变化,研究干旱胁迫和复水对萱草生理特性的影响。结果表明:(1)随着干旱胁迫的进行,轻度、中度以及重度干旱处理组的叶片相对含水量呈现逐渐下降趋势,复水后都有所恢复,但仍显著低于对照处理(P<0.05)。(2)叶绿素含量整体呈先升后降的变化趋势,复水后均增加,轻度、中度干旱胁迫处理的叶绿素含量与对照组差异均未达到显著水平(P>0.05)。(3)相对电导率均显著高于对照组(P<0.05)直至28 d,复水后各处理组的相对导电率均明显下降;随着胁迫时间的延长,轻度、中度、重度干旱处理下的丙二醛(MDA)含量都呈现逐渐增加的变化趋势,复水后各处理组都迅速下降,但均显著高于对照组(P<0.05)。(4)可溶性糖含量、脯氨酸含量在3个处理组中均呈持续增加的变化趋势,复水后各处理组的可溶性糖含量、脯氨酸含量均明显下降,但仍均显著高于对照组(P<0.05)。(5)各处理组的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性都有不同程度的增加,复水后轻度、中度、重度干旱处理的SOD、POD、CAT活性下降,但仍高于正常水平。

关键词: 萱草, 干旱胁迫, 复水, 渗透调节, 抗氧化酶

Abstract:

To study the effects of drought stress and rehydration on the physiological characteristics of day lily (Hemerocallis fulva), the changes of relative water content, chlorophyll, relative electrical conductivity, antioxidant enzyme activity and osmotic regulation substances in leaves were measured by water control in pot culture to simulate drought and rewatering. The results showed that: (1) with the progress of drought stress, the leaf relative water content in mild, moderate and severe drought treatments decreased gradually, and recovered after rewatering, but still significantly lower than that of the control treatment (P<0.05). (2) The chlorophyll content increased at first and then decreased, and increased after rewatering. The chlorophyll content of mild and moderate drought stress treatments was not significantly different from that of the control group (P>0.05). (3) The relative conductivity was significantly higher than that of the control group (P<0.05) until 28 days, and the relative conductivity of each treatment group decreased significantly after rehydration. With the extension of stress time, the content of MDA in mild, moderate and severe drought treatments increased gradually, and decreased rapidly after rewatering, but it was significantly higher than that in the control group (P<0.05). (4) Soluble sugar and proline increased continuously in the three treatment groups. After rehydration, the contents of soluble sugar and proline in all treatment groups decreased significantly, but they were still significantly higher than those in the control group (P<0.05). (5) The activities of SOD, POD and CAT in each treatment group increased in varying degrees. After rehydration, the activities of POD, SOD and CAT in mild, moderate and severe drought treatments decreased, but still higher than the normal level.

Key words: Hemerocallis fulva, drought stress, rehydration, osmoregulation, antioxidase