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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (18): 123-130.doi: 10.11924/j.issn.1000-6850.casb2022-0448

• 生物科学 • 上一篇    下一篇

贝莱斯芽孢杆菌(Bacillus velezensis)YFB3-1高密度液体发酵条件优化

鲁耀雄1,2(), 李魏2, 周瑚3, 瞿玉国4, 熊有明5, 王运生2()   

  1. 1 湖南省农业科学院农业环境生态研究所，长沙 410125
    2 湖南农业大学植物保护学院，长沙 410128
    3 岳阳市检验检测中心，湖南岳阳 414000
    4 古丈县特色产业服务中心，湖南古丈 416300
    5 湖南省烟草公司衡阳市公司，湖南衡阳 421099
  • 收稿日期:2022-06-06 修回日期:2022-08-15 出版日期:2023-06-25 发布日期:2023-06-25
  • 通讯作者: 王运生，男，1979年出生，湖南常宁人，硕士生导师，教授，博士，主要从事农业微生物基因组学研究。通信地址：410128 湖南省长沙市芙蓉区农大路1号植物保护学院，Tel：18773181264，E-mail：wyunsheng@gmail.com。
  • 作者简介:

    鲁耀雄，男，1981年出生，湖南永州人，副研究员，博士，主要从事农业废弃物资源化利用及相关微生物研究。通信地址：410125 湖南省长沙市芙蓉区马坡岭远大二路560号，Tel：13875906429，E-mail：。

  • 基金资助:
    湖南省自然科学基金项目“蚯蚓媒介Bacillus sp. YFB3-1缓解百合连作障碍病害发生的微生物机制研究”(2020JJ4393); 农业农村部废弃物肥料化利用重点实验开放基金“稻耳轮作制度下的农业废弃物高效循环利用技术研究与示范”(KLFAW202004); 长沙市自然科学基金项目“蚯蚓联合Bacillus velezensis YFB3-1对连作辣椒疫病的防治效果及机制研究”(kq2208105)

Bacillus velezensis YFB3-1: Optimization of Liquid Fermentation Conditions for High Density

LU Yaoxiong1,2(), LI Wei2, ZHOU Hu3, QU Yuguo4, XIONG Youming5, WANG Yunsheng2()   

  1. 1 Institute of Agri-Environment and Ecology, Hunan Academy of Agricultural Sciences, Changsha 410125
    2 College of Plant Protection, Hunan Agricultural University, Changsha 410128
    3 Yueyang Inspection and Testing Center, Yueyang, Hunan 414000
    4 Guzhang County Characteristic Industry Service Center, Guzhang, Hunan 416300
    5 Hengyang Company of Hunan Tobacco Corporation, Hengyang, Hunan 421099
  • Received:2022-06-06 Revised:2022-08-15 Online:2023-06-25 Published:2023-06-25

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摘要：

旨在获得高密度的贝莱斯芽孢杆菌(Bacillus velezensis)YFB3-1生防菌剂，用于预防作物土传病害的发生。采用单因素和正交试验对菌株YFB3-1的液体培养基和培养条件进行优化。在酵母粉8 g/L、复合氨基酸10 g/L、氯化钠1 g/L、硫酸镁(MgSO4·7H2O) 1 g/L的液体培养基中培养菌株YFB3-1，初始pH 7.0、接种量2.5%、发酵温度30℃、转速180 r/min和培养时间48 h为发酵条件时，获得的菌体浓度为2.89×1010 cfu/mL，是菌株YFB3-1液体发酵的最优培养基和培养条件，比牛肉膏蛋白胨液体培养基(NB)的菌体浓度4.77×109 cfu/mL，提高了5.06倍，在相同时间内可以提高发酵效率。本研究为生防菌B. velezensis YFB3-1的产业化扩大培养和推广应用提供了理论依据和技术参数。

关键词: 贝莱斯芽孢杆菌, 正交试验, 高密度液体发酵, 发酵条件优化

Abstract:

The aim is to obtain high density Bacillus velezensis YFB3-1 biocontrol agent which can be used to prevent soil-borne diseases of crops. In this study, single factor and orthogonal experiments were used to optimize the liquid medium and culture conditions of strain YFB3-1. The results showed that strain YFB3-1 was cultured in liquid medium with yeast powder 8 g/L, compound amino acid 10 g/L, sodium chloride 1 g/L and magnesium sulfate (MgSO4·7H2O) 1 g/L, and when the fermentation conditions were initial pH of 7.0, inoculation amount of 2.5%, fermentation temperature of 30℃, rotation speed of 180 r/min and culture time of 48 h, the bacterial concentration was 2.89×1010 cfu/mL, and the optimal culture medium and culture conditions were obtained for the liquid fermentation of strain YFB3-1. Compared with beef extract peptone liquid medium (NB), in which the bacterial concentration was 4.77×109 cfu/mL, the bacterial concentration under the fermentation conditions of this experiment increased by 5.06 times, and the fermentation efficiency could be improved in the same time. The study provides a theoretical basis and technical parameters for the industrial expansion and application of B. velezensis YFB3-1.

Key words: Bacillus velezensis, orthogonal test, high-density liquid fermentation, optimization of fermentation condition