欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (3): 123-130.doi: 10.11924/j.issn.1000-6850.casb2024-0003

• 生物科学 • 上一篇    下一篇

一株野生肉色隔孢伏革菌的分子鉴定、生物学特性及驯化

饶永斌1(), 张君丽2()   

  1. 1 三明市食用菌技术推广站，福建三明 365000
    2 西藏自治区农牧科学院蔬菜研究所，拉萨 850000
  • 收稿日期:2024-01-03 修回日期:2024-04-15 出版日期:2025-01-20 发布日期:2025-01-20
  • 通讯作者:
    张君丽，女，1979年出生，陕西合阳人，副研究员，硕士，主要从事野生菌资源收集鉴定利用与栽培示范研究。E-mail：。
  • 作者简介:

    饶永斌，男，1982年出生，福建泰宁人，农艺师，硕士研究生，主要从事食用菌新品种、新技术试验示范及推广工作。通信地址：365000 三明市三元区红岩新村37幢201，三明市食用菌技术推广站，Tel：0598-8225730，18960580968，E-mail：。

  • 基金资助:
    西藏自治区科学技术厅重点研发项目“特色食用菌种质资源创新研究与菌种繁育关键技术集成示范”(XZ202001ZY0041N)

Molecular Identification, Biological Characteristics and Domestication of A Wild Strain of Peniophora incarnata

RAO Yongbin1(), ZHANG Junli2()   

  1. 1 Sanming Edible Fungi Technology Promotion Station, Sanming, Fujian 365000
    2 Vegetable Research Institute, Tibet Academy of Agriculture and Animal Husbandry Sciences, Lhasa 850000
  • Received:2024-01-03 Revised:2024-04-15 Published:2025-01-20 Online:2025-01-20

RichHTML

1

PDF (PC)

36

摘要：

本研究旨在开发利用西藏野生蕈食用菌资源，通过对采集自波密县扎木镇编号为X21108的菌株进行分离鉴定，采用ITS序列分析并运用最大似然法构建系统发育树，确定该菌株为肉色隔孢伏革菌(Peniophora incarnata)。以菌丝生长速度作为指标，对菌株最适碳源、氮源、pH和温度4个方面进行探究，以研究该菌株的生物学特性。结果表明，在生物学特性方面，X21108菌株菌丝体最适碳源为蔗糖，该碳源条件下菌丝生长速度达到6.93 ± 0.38 mm/d；以硝酸铵为氮源时，肉色隔孢伏革菌菌丝生长速度最快，达到7.35 ± 0.22 mm/d；最佳pH和温度分别5.0和25℃，菌丝生长速度分别为6.05 ± 0.42 mm/d和8.22 ± 0.54 mm/d。生物学特性研究表明肉色隔孢伏革菌X21108是生长环境偏酸性的中温型菌株。在驯化方面，在组成为粗木屑40%、细木屑20%、棉籽壳20%、麦麸18%、石灰1%、蔗糖1%的培养料上栽培，培养10 d形成原基，50 d可出菇，获得肉色隔孢伏革菌子实体。综上所述，本研究对西藏野生蕈菌资源进行分子鉴定、生物学特性研究和驯化栽培，对野生食用菌资源的开发利用具有重要的现实意义。

关键词: 西藏野生蕈菌, 肉色隔孢伏革菌, ITS序列分析, 分子鉴定, 系统发育树, 生物学特性, 驯化栽培

Abstract:

In order to develop the utilization of wild edible mushroom resources in Tibet, this study isolated and identified a strain coded X21108 collected from Zhamu Town, Bomê County, Tibet. Based on ITS sequence analysis and the construction of a phylogenetic tree using the maximum likelihood method, the strain was identified as Peniophora incarnata. The mycelial growth rate was used as an indicator to investigate the optimal carbon source, nitrogen source, pH, and temperature for the strain in order to study its biological characteristics. The results indicated that in terms of biological characteristics the optimal carbon source for the mycelial growth of this strain was sucrose, under which the mycelial growth rate reached 6.93 ± 0.38 mm/d; using ammonium nitrate as the nitrogen source, the mycelial growth rate of Peniophora incarnata was the fastest, reaching 7.35 ± 0.22 mm/d; the optimal pH and temperature were 5.0 and 25℃, with the mycelial growth rates being 6.05±0.42 mm/d and 8.22 ± 0.54 mm/d, respectively. The results of biological characteristics showed that Peniophora incarnata X21108 was a medium-temperature strain with acidic growth environment. In terms of domestication, the formation of primordia was formed after 10 days of cultivation on a substrate consisting of coarse wood chips (40%), fine wood chips (20%), cottonseed hulls (20%), wheat bran (18%), lime (1%), and sucrose (1%), and mushrooms could be harvested after 50 days, resulting in the formation of Peniophora incarnata fruiting bodies. In summary, this study on the molecular identification, biological characteristics, and domestication cultivation of wild mushroom resources in Tibet had important practical significance for the development and utilization of wild edible mushroom resources.

Key words: Tibetan wild edible fungi, Peniophora incarnata, ITS sequence analysis, molecular identification, the phylogenetic tree, biological characteristics, domestication cultivation