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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (9): 1-7.doi: 10.11924/j.issn.1000-6850.casb2024-0491

• 农学·农业基础科学 •    下一篇

氧化石墨烯引发对盐胁迫下水稻种子萌发的影响

梁文召1(), 周诗意1, 韦瑞燕1, 石琳亚2, 刘乃新1(), 于清涛3   

  1. 1 黑龙江大学现代农业与环境学院，哈尔滨 150080
    2 哈尔滨师范大学生命科学与技术学院，哈尔滨 150028
    3 哈尔滨市农业科学院、，哈尔滨 150028
  • 收稿日期:2024-07-19 修回日期:2024-09-15 出版日期:2025-03-25 发布日期:2025-03-25
  • 通讯作者:
    刘乃新，女，1980年出生，黑龙江哈尔滨人，副研究员，博士，研究方向：糖料作物质量安全及营养品质评价。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学农学楼，Tel：13504511205，E-mail：。
  • 作者简介:

    梁文召，女，1996年出生，甘肃渭源人，研究生，研究方向：水稻萌发及抗倒伏。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学农学楼，Tel：15095460739，E-mail：。

  • 基金资助:
    财政部和农业农村部国家现代农业产业技术体系（糖料）建设项目“质量安全与营养品质评价”(CARS-170503); 黑龙江省博士后基金“纳米硒生物强化调控寒地香稻产量和品质的分子机制研究”(LBH-Z22245)

Effect of Graphene Oxide on Rice Seed Germination Under Salt Stress

LIANG Wenzhao1(), ZHOU Shiyi1, WEI Ruiyan1, SHI Linya2, LIU Naixin1(), YU Qingtao3   

  1. 1 School of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
    2 College of Life Science and Technology, Harbin Normal University, Harbin 150028
    3 Harbin Academy of Agricultural Sciences, Harbin 150028
  • Received:2024-07-19 Revised:2024-09-15 Published:2025-03-25 Online:2025-03-25

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摘要：

为了研究盐胁迫下氧化石墨烯(graphene oxide，GO)处理对水稻种子萌发的影响，本试验以水稻‘哈粳稻15号’为试验材料，在正常条件和盐胁迫下(0.05 mol/L NaCl)采用不同质量浓度(0、25、50、100 mg/L)的GO引发处理，检测不同处理后水稻种子的抗氧化酶活性、丙二醛及其渗透调节物含量的变化。结果表明：（1）低浓度GO(25 mg/L)可以促进水稻种子的萌发，而高浓度GO(50、100 mg/L)则明显抑制其萌发。25 mg/L的GO可以增加盐胁迫下种子的生命力，提高出苗率；（2）高浓度GO与NaCl复合处理，可以进一步加重盐胁迫对水稻种子生长的抑制效应，同时提高抗氧化酶活性，降低丙二醛含量，减轻氧化应激反应；（3）GO处理促进了渗透调节物的合成与积累，增加水稻种子的渗透调节能力和抗盐害能力，强化幼苗的耐盐能力，显著提升其在盐环境下的出苗和成苗效率。低浓度GO可以促进水稻萌发，而高浓度GO对水稻生长具有一定危害。特别是在盐胁迫下，高浓度GO可以加大盐胁迫对水稻生长的毒害。建议盐碱地区在水稻生产实践中慎用高浓度GO相关肥料等。

关键词: 氧化石墨烯, 盐胁迫, 水稻, 种子引发, 萌发

Abstract:

To explore the effect of graphene oxide (GO) treatment on rice seed germination under salt stress, this experiment used the rice variety 'Harbin Japonica Rice 15' as the experimental material. Under normal conditions and salt stress (0.05 mol/L NaCl), GO was induced at different mass concentrations (0, 25, 50, 100 mg/L) to detect the changes in antioxidant enzyme activity, malondialdehyde, and osmotic regulator content of rice seeds after different treatments. The results show that: (1) low concentrations of GO (25 mg/L) can promote the germination of rice seeds, while high concentrations of GO (50, 100 mg/L) significantly inhibit their germination. 25 mg/L GO can increase seed vitality and improve germination rate under salt stress; (2) high concentrations of GO and NaCl combined treatment can further exacerbate the inhibitory effect of salt stress on rice seed growth, increase antioxidant enzyme activity, reduce malondialdehyde content, and alleviate oxidative stress response; (3) GO treatment promotes the synthesis and accumulation of osmotic regulators, increases the osmotic regulation ability and salt resistance of rice seeds, strengthens the salt tolerance of seedlings, and significantly improves their emergence and seedling efficiency in salt environments. Low concentration GO can promote rice germination, while high concentration GO has certain harm to rice growth. Especially under salt stress, high concentrations of GO can increase the toxicity of salt stress on rice growth. It is suggested that high concentration GO related fertilizers should be used with caution in rice production practice in saline alkali areas.

Key words: graphehe oxide, salt stress, Oryza sativa L., seed priming, germination